关键词: 婴幼儿配方乳粉；3-氯-1,2-丙二醇酯；缩水甘油酯；气相色谱-质谱法；酸水解

Abstract: An analytical method for the determination of 3-monochloropropane-1,2-diol esters (3-MCPDEs) and glycidyl esters (GEs) in infant formula milk powder was established using gas chromatography-mass spectrometry (GC-MS). Based on optimized lipid extraction and derivatization conditions, the method was evaluated for spiked recovery, linear relationship, precision, limit of detection (LOD) and limit of quantification (LOQ). Quantification was carried out using the internal standard method. The proposed method showed good linearity in the range of 5–500 ng (r > 0.999), with an LOD of 0.006 mg/kg and an LOQ of 0.015 mg/kg. The recovery rates at three spiked concentration levels of 10, 100 and 500 ng ranged from 95.6% to 100.9%, with relative standard deviations (RSDs) less than 5.03%. The results of GC-MS and gas chromatography-tandem mass spectrometry (GC-MS/MS) for 3-MCPDEs and GEs in several batches of infant formula samples did not significantly differ. In addition, compared with GC-MS/MS, the GC-MS method was simpler and easier to operate with lower cost and could be promoted in different levels of laboratories. Therefore, this method is suitable for determining the content of 3-MCPDEs and GEs in infant formula milk powder.

Key words: infant formula milk powder; 3-monochloropropane-1,2-diol esters; glycidyl esters; gas chromatography-mass spectrometry; acid hydrolysis