关键词: 乳杆菌; 酸奶; 黏附; HeLa细胞; 过氧化氢

Abstract: Probiotic Lactobacillus strains were isolated from yogurt, cultured on de Man, Rogosa and Sharpe (MRS) agar and identified according to their 16S rRNA sequences. The Lactobacilli strains were assessed for hydrogen peroxide (H2O2) production and adhesion to HeLa cells to determine their potential for preventing infections of the female reproductive tract. A total of 10 isolates were identified including 5 L. acidophilus strains, 1 L. paracasei strain, 1 L. helveticus strain, 1 L. rhamnosus strain, 1 L. delbrueckii strain and 1 L. plantarum strain. The L. helveticus strain was strongly adherent to HeLa cells and demonstrated high H2O2 production, whereas other 9 strains had a relatively low ability to produce H2O2. Although most of the probiotic Lactobacillus strains isolated from yogurt did not have biochemical characteristics in relation to preventing infections of the female reproductive tract, the L. helveticus strain might have a protective effect on female urogenital health.

Key words: Lactobacillus；yogurt；adhesion；HeLa cell；H2O2