关键词: 副干酪乳杆菌; 通便; 小鼠; 便秘

Abstract: Lactobacillus paracasei subsp. paracasei LC-01 is a probiotic strain which has strong antioxidant and immunoregulatory activities. This study was designed to evaluate the laxative effect of strain LC-01 in constipated mice. Male Balb/c mice were randomly divided into blank group, model control group, positive control group and three dose groups. Both the blank and model control groups were administered orally with 10% skim milk at a dose of 0.1 mL/(kg·d) once a day. Lactobacillus casei subsp. casei Shirota (1 × 108 CFU/mL) and Lactobacillus casei subsp. casei LC-14 (1 × 106, 1 × 108 CFU/mL and 1 × 1010 CFU/mL) were used as control strains (also at 0.1 mL/(kg·d)). Strain LC-01 suspensions at concentrations of 1 × 106, 1 × 108 CFU/mL and 1 × 1010 CFU/mL were given to mice at the same dose as 10% skim milk. Constipation was induced in mice by compound diphenoxylate. After 7 days of administration of strain LC-01, the first defecation time of mice was shortened, and the number, weight and water content of stools were increased markedly. After another 7 days, the ink propelling rate became much higher. These results indicate that strain LC-01 has an outstanding laxative effect in constipated mice.

Key words: Lactobacillus paracasei；defecation；mice；constipation