关键词: 生物芯片, 生乳, 高效液相色谱-串联质谱法, 质量安全

Abstract: In order to verify the detection effect of biochip technology in the detection of veterinary drugs in raw milk and promote its application, 80 kinds of common veterinary drugs were detected by biochip rapid detection method, and confirmed by high performance liquid chromatography-tandem mass spectrometry. The results showed that the detection limit of the biochip method met the requirements of the national standard, and some of them were better than the national standard. The linear range of the method was wide, the correlation coefficient ( r ) was > 0.99, and there was a good correlation in the linear range. Through the recovery of negative samples, the standard samples with 2 times and 4 times the detection limit were determined. The recovery rate was 70 % ~ 130 %, and the coefficient of variation ( CV value ) was 5 % ~ 10 %.The method has the advantages of simple pretreatment, short detection cycle, high sensitivity and large flux, which is suitable for rapid screening of large quantities of samples. Using the above methods, 100 batches of raw milk collected from Shanghai area from 2022 to 2023 were detected, and 10 batches of raw milk containing veterinary drug residues were detected, including 4 batches of streptomycin ( detection value 5.86~10.26 μg/kg ), 3 batches of sulfapyridine ( detection value 1.09~1.42 μg/kg ), 2 batches of sulfamonomethoxine ( detection value 1.38~1.46 μg/kg ), and 1 batches of avermectin ( detection value 1.75 μg/kg ).The above detection values are far lower than the announcements of international organizations, the Ministry of Agriculture and Rural Affairs or the national standard limit regulations. The quality and safety of raw milk in this city is generally good. Although these veterinary drugs have been detected, they will not cause harm to the human body.

Key words: biochip, raw milk, High performance liquid chromatography-tandem mass spectrometry, quality and safety