Whole Genome Sequencing and Safety Evaluation of Lactiplantibacillus plantarum SC-1

doi:10.7506/rykxyjs1671-5187-20260220-015

Journal of Dairy Science and Technology ›› 2026, Vol. 49 ›› Issue (3): 11-16.DOI: 10.7506/rykxyjs1671-5187-20260220-015

• Basic Research • Previous Articles Next Articles

Whole Genome Sequencing and Safety Evaluation of Lactiplantibacillus plantarum SC-1

WU Zhongling

Lactiplantibacillus plantarum SC-1, a strain able to promote the production of ergosterol by Monascus isolated from naturally fermented Chinese sauerkraut, was evaluated for genetic characteristics and application safety. Whole genome sequencing and Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) annotation showed that the functional genes of strain SC-1 are involved in pathways including amino acid synthesis, carbon metabolism, genetic information regulation, and environmental stress response. The carbohydrate-active enzymes (CAZy) of this strain were predominantly glycoside hydrolases and glycosyltransferases, indicating that the strain can metabolize carbohydrates. Acid and bile salt tolerance assays demonstrated that the survival rate of SC-1 was (68.89 ± 3.91)% in an acidic environment (pH 2.5) and (50.30 ± 4.25)% at 0.3% (m/m) bile salt content, respectively. In addition, SC-1 exhibited γ-hemolysis (non-hemolytic activity) and it was sensitive to common antibiotics such as penicillin but showed resistance to several aminoglycoside antibiotics. SC-1 had no significant virulence and its resistance genes posed no transfer risk.

Published:2026-07-06

植物乳植杆菌SC-1全基因组测序及安全性评价

伍忠玲

光明乳业股份有限公司乳业研究院，上海乳业生物工程技术研究中心，农业农村部功能乳品加工重点实验室，乳业生物技术国家重点实验室，上海 201103

基金资助:
上海市国资委企业创新发展与能级提升项目（2022013）

Abstract

Abstract: Lactiplantibacillus plantarum; whole genome sequencing; safety evaluation; functional genes

摘要： 对一株分离自天然酸菜?可促进红曲霉产麦角甾醇的植物乳植杆菌(Lactiplantibacillus plantarum)SC-1 的遗传特征与应用安全性进行研究?通过对SC-1菌株进行全基因组测序,结合京都基因与基因组百科全书及基因本体论数据库注释,得出SC-1菌株功能基因参与氨基酸合成?碳代谢?遗传信息调控及环境应激响应等通路?碳水化合物活性酶数据库注释表明,SC-1菌株的碳水化合物活性酶以糖苷水解酶和糖苷转移酶为主,具备碳水化合物代谢能力?耐酸?耐胆盐实验表明,SC-1菌株在pH 2.5酸性环境和0.3%(m/m)胆盐环境中的存活率分别达(68.89±3.91)%和(50.30±4.25)%?此外,SC-1菌株为γ-溶血,无溶血性,对青霉素等常用抗生素敏感,对部分氨基糖苷类抗生素耐药?毒力因子与抗生素抗性基因分析表明,SC-1菌株无明显毒力作用,抗性基因无转移风险?

关键词: 植物乳植杆菌; 全基因组测序; 安全性评价; 功能基因

CLC Number:

TS201.3

State Key Laboratory of Dairy Biotechnology, Key Laboratory of Functional Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy and Food Co. Ltd., Shanghai 201103, China. Whole Genome Sequencing and Safety Evaluation of Lactiplantibacillus plantarum SC-1[J]. Journal of Dairy Science and Technology, 2026, 49(3): 11-16 https://doi.org/10.7506/rykxyjs1671-5187-20260220-015

伍忠玲. 植物乳植杆菌SC-1全基因组测序及安全性评价[J]. 乳业科学与技术, 2026, 49(3): 11-16 https://doi.org/10.7506/rykxyjs1671-5187-20260220-015

[1]	GAO Jiali, WANG Xiaoru, XIAO Xiao, CHEN Jia, MENG Deshang, SUN Wei. Identification of Peptoniphilus asaccharolyticus Isolated from Spoiled Fermented Milk Intended for Students [J]. Journal of Dairy Science and Technology, 2024, 47(5): 7-11.
[2]	LI Yue, REN Dayong. Research Progress on Isolation, Preparation and Functional Characteristics of Postbiotics and Their Applications in Dairy Products [J]. Journal of Dairy Science and Technology, 2024, 47(3): 61-70.
[3]	WU Zhongling, GAO Hongyan, LIU Zhenmin. Isolation and Identification of Lactobacillus plantarum and Its Effect on Pigment Production by Monascus [J]. Journal of Dairy Science and Technology, 2023, 46(5): 1-9.
[4]	ZHANG Yalun, WANG Zan, ZHANG Rui, CHEN Boxu, ZHOU Wei, ZHANG Yan. Quantitative Detection of Acetobacter aceti in Fermented Milk by Droplet Digital Polymerase Chain Reaction [J]. Journal of Dairy Science and Technology, 2023, 46(2): 13-17.
[5]	ZHANG Yalun, LI Yongbo, ZHANG Tao, CHEN Chen, ZHANG Jie, ZHOU Wei, ZHANG Yan. Establishment of a Helicase-Dependent Isothermal DNA Amplification Method for Rapid Detection of Lactobacillus plantarum in Fermented Milk [J]. Journal of Dairy Science and Technology, 2023, 46(1): 30-34.
[6]	ZHANG Yalun, YANG Fan, ZHANG Tao, ZHANG Rui, LI Xian, ZHOU Wei, ZHANG Yan. Development of Quality Control Samples for Qualitative Analysis of Listeria monocytogenes by Time-of-Flight Mass Spectrometry [J]. Journal of Dairy Science and Technology, 2022, 45(6): 27-32.
[7]	JIAO Yangbo, ZHAO Jie. Recent Development in Research on Lactic Acid Bacterial DNA Methylation [J]. Journal of Dairy Science and Technology, 2022, 45(5): 32-37.
[8]	ZHAO Xuanying, YANG Yang, WANG Guojiao, YONG Jingyi, LI Nan. Recent Progress in Postbiotics and Their Applications in Dairy Products [J]. Journal of Dairy Science and Technology, 2022, 45(2): 47-54.
[9]	YANG Youxin, WANG Ruixue, WANG Junguo. Progress in Research on the Protective Mechanism of Trehalose on Lactic Acid Bacteria [J]. Journal of Dairy Science and Technology, 2021, 44(6): 45-50.
[10]	YANG Ling, ZHANG Dong, QI Shihua, MA Xinying, ZHOU Shuaikang, AI Lianzhong, WANG Shijie. Process Optimization for Production of Freeze-Dried Cells of Bifidobacterim bifidum TMC3115 [J]. Journal of Dairy Science and Technology, 2021, 44(5): 12-17.
[11]	ZHAO Le, MENG Xiangchen. A Review of the Application of Transcriptome Sequencing in Research on Bacteriocin Synthesis by Lactobacillus plantarum [J]. Journal of Dairy Science and Technology, 2021, 44(5): 32-37.
[12]	ZHANG Tao, ZHANG Rui, ZHANG Yalun, XU Miaomiao, WANG Hui, CHEN Chen, ZHOU Wei, SHI Guohua. Development of Ready-to-Use Quality Control Samples for the Detection of Salmonella in Formula Foods for Special Medical Purposes Containing Milk [J]. Journal of Dairy Science and Technology, 2021, 44(4): 29-33.
[13]	ZHENG Huajie, WANG Qingyun, WANG Xin, WANG Zhandong. Progress in the Development of Growth-Promoting Materials for Lactic Acid Bacteria [J]. Journal of Dairy Science and Technology, 2020, 43(6): 46-50.
[14]	YAN Danli, WU Junrui, SHI Haisu, LUO Xue, YUE Xiqing, WU Rina. Recent Progress in a Next Generation Probiotic, Bacteroides ovatus [J]. Journal of Dairy Science and Technology, 2020, 43(1): 50-54.
[15]	WANG Shibo, ZHANG Jinlan, LI Pinglan. Isolation and Identification of Antioxidant Peptide from Culture Supernatant of Biifdobacterium [J]. Journal of Dairy Science and Technology, 2019, 42(4): 10-15.

Whole Genome Sequencing and Safety Evaluation of Lactiplantibacillus plantarum SC-1

植物乳植杆菌SC-1全基因组测序及安全性评价

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics