Abstract: This study focuses on the identification of Peptoniphilus asaccharolyticus from spoiled fermented milk intended for students based on isolation, counting, morphological observation, biochemical identification, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and whole-genome sequencing. The results showed that the isolate grew well on PCA medium under anaerobic conditions and was identified as P. asaccharolyticus on a fully automated microbial identification system with a confidence level of 98%. By MALDI-TOF MS, it was confirmed as P. sasaccharolyticus with a comparison value of 2.241. The result of whole genome sequencing showed an average nucleotide identity (ANI) of 98.58%. The proposed method allows rapid and accurate identification of P. sasaccharolyticus and can meet the requirements of food safety detection.

Key words: fermented milk intended for students; Peptoniphilus asaccharolyticus; strain identification; matrix-assisted laser desorption ionization time-of-flight mass spectrometry; whole genome sequencing

摘要： 以学生饮用乳（发酵乳）来源的不解糖嗜胨菌为研究对象，基于分离计数、形态学观察、生化鉴定、基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption ionization-time-of-flight mass spectrometry，MALDI-TOF MS）和全基因组测序技术，建立适用于不解糖嗜胨菌的鉴定方法。结果表明：不解糖嗜胨菌在PCA培养基上厌氧环境下生长较适；在全自动微生物鉴定系统上鉴定为不解糖嗜胨菌，置信度98%；MALDI-TOF MS鉴定为不解糖嗜胨菌，鉴定比对值为2.241。通过全基因组测序，平均核苷酸一致性分析结果为98.58%。利用本研究建立的方法能够满足不解糖嗜胨菌的快速、准确鉴定，符合食品安全检测需求。

关键词: 学生饮用乳（发酵乳）；不解糖嗜胨菌；菌株鉴定；基质辅助激光解吸电离飞行时间质谱；全基因组测序