Effect of Different Protease Treatments on the Solubility of Milk Protein Concentrate

doi:10.7506/rykxyjs1671-5187-20250710-045

Journal of Dairy Science and Technology ›› 2025, Vol. 48 ›› Issue (6): 8-14.DOI: 10.7506/rykxyjs1671-5187-20250710-045

Effect of Different Protease Treatments on the Solubility of Milk Protein Concentrate

Effect of the Interaction of Ferulic Acid with β-Lactoglobulin-Docosahexaenoic Acid Complex on β-Lactoglobulin Allergenicity

DU Yaxuan, ZHAO Chuan, ZHENG Xinyue, MAO Jiayi, XU Yizhou, LU Yuhang, CHEN Daichang, ZHAO Qian, ZHANG Meng, SHA Yicheng, LIANG Xiaona

Published:2026-01-15

阿魏酸与β-乳球蛋白-DHA复合物的相互作用对β-乳球蛋白致敏性的影响

杜雅宣，赵川，郑欣玥，毛佳怡，许一舟，陆宇航，陈代昌，赵倩，张萌，沙祎铖，梁肖娜

（淮阴师范学院生命科学学院，江苏淮安 223300）

基金资助:
大学生创新训练计划项目（X202510323030；X202510323109）

Abstract

Abstract: (School of Life Science, Huaiyin Normal University, Huai’an 223300, China)

摘要： β-乳球蛋白（β-lactoglobulin，BLG）是牛乳中的主要过敏原，可引起严重过敏反应，通过阿魏酸（ferulic acid，FA）协同二十二碳六烯酸（docosahexaenoic acid，DHA）修饰BLG构建三元复合物，探究对其抗原性和结构特性的影响。采用非共价结合方式获得BLG-DHA-FA复合物，通过多光谱技术、分子对接技术和间接竞争酶联免疫吸附测定法对复合物进行结构表征和抗原性研究。结果表明，FA与BLG-DHA结合后改变了芳香族氨基酸的微环境，极性增强；FA、DHA通过疏水相互作用及氢键结合于BLG的疏水空腔内，并使BLG的二级结构改变，β-折叠和无规卷曲减少，β-转角增加；与BLG、BLG-DHA相比，加入FA可显著降低抗原性，当FA质量浓度为0.08 mg/mL时免疫球蛋白G结合能力抑制率最高，达到40%。

关键词: β-乳球蛋白；二十二碳六烯酸；阿魏酸；互作效应；致敏性

CLC Number:

TS252.1

DU Yaxuan, ZHAO Chuan, ZHENG Xinyue, MAO Jiayi, XU Yizhou, LU Yuhang, CHEN Daichang, ZHAO Qian, ZHANG Meng, SHA Yicheng, LIANG Xiaona. Effect of Different Protease Treatments on the Solubility of Milk Protein Concentrate[J]. Journal of Dairy Science and Technology, 2025, 48(6): 8-14.

杜雅宣，赵川，郑欣玥，毛佳怡，许一舟，陆宇航，陈代昌，赵倩，张萌，沙祎铖，梁肖娜. 阿魏酸与β-乳球蛋白-DHA复合物的相互作用对β-乳球蛋白致敏性的影响[J]. 乳业科学与技术, 2025, 48(6): 8-14.

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Effect of Different Protease Treatments on the Solubility of Milk Protein Concentrate

阿魏酸与β-乳球蛋白-DHA复合物的相互作用对β-乳球蛋白致敏性的影响

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