Journal of Dairy Science and Technology ›› 2026, Vol. 49 ›› Issue (3): 17-24.DOI: 10.7506/rykxyjs1671-5187-20260220-014

• Biotechnology • Previous Articles    

Inhibitory Effect of Bifidobacterium longum subsp. longum BD3150 on Enterohemorrhagic Escherichia coli O157:H7

WANG Yitian, WANG Jing, REN Quanlu, WU Zhengjun   

  1. This study systematically investigated the inhibitory activity of Bifidobacterium longum subsp. longum BD3150 and its cell-free supernatant (CFS) against enterohemorrhagic Escherichia coli O157:H7 (EHEC O157) using the co-culture assay and the agar diffusion method. When 2’-fucosyllactose (2’-FL) served as the sole carbon source, the relative abundance of EHEC O157 after 6 and 18 h of co-culture with B. longum subsp. longum BD3150 was 72.3% and 67.8%, respectively, which was lower than that observed using glucose as the sole carbon source. The antibacterial activity of CFS from B. longum subsp. longum BD3150, cultivated in modified de Man-Rogosa-Sharpe (MRS) medium, was further characterized. The optimum culture conditions were found to be an inoculation size of 3.0%, incubation temperature of 37 ℃, and incubation time of 36 h. A negative correlation was observed between the diameter of the inhibition zone and the pH of the CFS. Furthermore, by adjusting the pH of the CFS and performing centrifugal ultrafiltration (3 kDa, 7 500×g for 25 min), it was revealed that the inhibitory activity against EHEC O157 was attributed to organic acids.
  • Published:2026-07-06

长双歧杆菌长亚种BD3150对肠出血性大肠杆菌 O157:H7活性的抑制作用

王禕天, 王晶, 任全路, 吴正钧   

  1. 光明乳业股份有限公司乳业研究院,上海乳业生物工程技术研究中心,农业农村部功能乳品加工重点实验室, 乳业生物技术国家重点实验室,上海 201103
  • 基金资助:
    “十四五”国家重点研发计划重点专项（2022YFD2100705）；上海市国资委企业创新发展与能级提升项目（2022013）

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Abstract: Bifidobacterium; antibacterial activity; agar diffusion method; cell free supernatant; human milk oligosaccharides

摘要： 采用共培养和琼脂扩散法评价长双歧杆菌长亚种BD3150及其无细胞发酵上清液对肠出血性大肠杆菌 O157:H7(enterohemorrhagic Escherichia coli O157:H7,EHEC O157)生长的抑制作用?结果表明,以2’-岩藻糖基乳糖作为唯一碳源,共培养6?18 h后EHEC O157相对丰度较以葡萄糖为碳源下降至72.3%和67.8%?进一步研究菌株长双歧杆菌长亚种BD3150在改良MRS培养基中所得发酵上清液的抑菌特性,确定其最佳制备条件为接种量3.0%?发酵温度37 ℃?发酵时间36 h,同时发现抑菌圈直径与上清液pH值呈负相关?通过调节无细胞发酵上清液的pH值和超滤离心(3 kDa?7 500×g?25 min),发现无细胞发酵上清液中主要抑菌物质为有机酸?

关键词: 双歧杆菌; 抑菌活性; 琼脂扩散法; 无细胞上清液; 母乳寡糖

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