Abstract: Water-soluble vitamins, as essential micronutrients for the human body, play a vital role in maintaining normal physiological functions, and their deficiency may lead to metabolic disorders and related health issues. Infant formula is fortified with these vitamins through nutrient fortification technologies to support the healthy growth and development of infants and young children. Due to the matrix complexity of infant formula and the instability of water-soluble vitamins, it is a great challenge to accurately analyze and detect multiple water-soluble vitamins in infant formula. In this paper, we review the latest developments in extraction and separation (protein precipitation, acid hydrolysis, and enzymatic hydrolysis) and detection (microbiological method, liquid chromatography, and liquid chromatography-mass spectrometry) techniques for water-soluble vitamins in infant formula, and we discuss future prospects in this field. Through this review, we aim to provide a reference for relevant researches.

Key words: water-soluble vitamins; infant formula milk powder; extraction and separation techniques; detection methods

摘要： 水溶性维生素作为人体必需的微量营养元素，对维持正常的生理功能具有重要作用，其缺乏可能导致代谢紊乱及相关的健康问题。婴幼儿配方乳粉通过营养强化技术科学添加这类维生素，旨在满足婴幼儿的生长发育需要，支持其健康成长与生理功能发育。由于婴幼儿配方乳粉基质的复杂性和水溶性维生素的不稳定性，对婴幼儿配方乳粉中的多种水溶性维生素进行精准分析检测是一个巨大的挑战。本文阐述近几年婴幼儿配方乳粉中水溶性维生素的蛋白质沉淀法、酸解法和酶解法等提取分离技术以及微生物法、液相色谱法和液相色谱-质谱法等检测方法的最新研究进展，并对目前各个提取及分离技术和相关检测方法进行探讨和展望，以期为相关研究提供参考和依据。

关键词: 水溶性维生素；婴幼儿配方乳粉；提取及分离技术；检测方法