乳业科学与技术 ›› 2023, Vol. 46 ›› Issue (4): 55-60.DOI: 10.7506/rykxyjs1671-5187-20230801-038

• 专题论述 • 上一篇    下一篇

基于DNA分析的特色乳掺假检测技术研究进展

王 越,张奕南,叶 青,刘 洋   

  1. 上海市质量监督检验技术研究院,国家市场监管重点实验室(乳及乳制品检测与监控技术),上海 200233
  • 出版日期:2023-07-01 发布日期:2024-01-26

Research Progress on DNA-based Technologies for Adulteration Detection in Non-bovine Milk

WANG Yue, ZHANG Yinan, YE Qing, LIU Yang   

  1. Shanghai Institute of Quality Inspection and Technical Research, Key Laboratory of Milk and Dairy Products Detection and Monitoring Technology for State Market Regulation, Shanghai 200233, China
  • Published:2023-07-01 Online:2024-01-26

摘要: 近年,特色乳中检出非标称物质的舆情时有爆出,特色乳的掺假检测是保证市场监管的重要技术支撑。DNA分析检测技术是食品真实性鉴别中常见且有效的手段,在特色乳的掺假检测中也具有优势。本文就特色乳概况及常用的DNA检测技术在特色乳掺假检测中的应用进行综述:从标记DNA靶基因出发,按照定量与定性的需求进行分类,分析不同技术的原理,并以近年在特色乳检测中的具体应用为例,对目的基因、检测方法、灵敏度和应用场景进行介绍,探讨各种检测技术的优势与局限性,以期为特色乳的质量把控提供参考。

关键词: 特色乳;掺假检测;DNA分析技术;DNA靶基因;定量检测

Abstract: In recent years, it has been often reported that non-nominal ingredients are found in milks from minor dairy species. Adulteration detection in milks from minor dairy species provides important technical support for market surveillance. DNA-based detection is a common and effective approach for food authentication, and also has advantages in milk adulteration detection. In order to provide a reference for the quality control of minor species milks, this article reviews the general aspects of minor species milks and the application of common DNA-based qualitative and quantitative techniques in adulteration detection in minor species milks in term of target gene selection, and their principles, sensitivities, application scenarios, advantages and limitations.

Key words: minor species milks; adulteration detection; DNA-based technique; target genes; quantitative detection

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