关键词: 蛋氨酸;奶牛乳腺上皮细胞;乳糖;β-酪蛋白;甘油三酯;细胞增殖

Abstract: An in vitro model of dairy cow mammary epithelial cells (DCMECs) was developed and used to study the relationship between methionine and milk protein synthesis or the lactation ability of the mammary gland. After 24 hours of in vitro culture in the presence of various concentrations (0, 0.2, 0.4, 0.8, 1.2 mmol/L and 1.6 mmol/L) of methionine, the lactation ability of DCMECs was analyzed by high performance liquid chromatography (HPLC), real-time quantitative PCR (RT-PCR), cell viability analyzer and triglyceride kit. It was found that methionine at 1.2 mmol/L had the most significant proliferationpromoting effect on DCMECs during the culture period. In addition, the highest amounts of lactose secretion, β-casein mRNA expression and triglyceride synthesis were achieved.

Key words: methionine；dairy cow mammary epithelial cells；lactose；β-casein；triglyceride；cell proliferation