乳业科学与技术 ›› 0, Vol. ›› Issue (): 0-0.

• 基础研究 •    下一篇

基于代谢组学的乳酸乳球菌乳酸亚种豆乳发酵特性分析

何海1,杨宇1,周思思1,刘小鸣2   

  1. 1. Jiangnan University
    2. Xiaoming Liu, Professor, Jiangnan University
  • 收稿日期:2024-04-22 修回日期:2024-07-04 出版日期:2024-05-01 发布日期:2024-08-26
  • 通讯作者: 刘小鸣

Analysis of fermentation characteristics of Lactococcus lactis subsp. lactis in soymilk based on metabolomics

  • Received:2024-04-22 Revised:2024-07-04 Published:2024-05-01 Online:2024-08-26

摘要: 目前,国内外关于乳酸乳球菌乳酸亚种的研究主要集中于在牛乳发酵中的应用,豆乳发酵中的应用研究较少。本文采用发酵指标、代谢组学和感官评价,对乳酸乳球菌乳酸亚种菌株在豆乳中的发酵特性进行了分析。结果表明,具有优良蔗糖代谢特性的DYNDL21-2、DYNDL1-2和FJNDD18M8这三种菌株展现了良好的生长和产酸能力。在乳酸乳球菌乳酸亚种发酵豆乳中,己醛和多种呋喃类杂环化合物的丰度显著下调,有效降低了豆腥味的强度。菌株FJNDD18M8发酵豆乳中乙醛、乙偶姻及2,3-丁二酮等物质的显著上调,提高了产品的奶香和奶油风味,进而改善了豆乳风味。

关键词: 乳酸乳球菌乳酸亚种, 豆乳, 发酵特性, 代谢组

Abstract: The fermentation behavior of Lactococcus lactis subsp. lactis in soymilk was analyzed by fermentation index, metabolomics and sensory evaluation. The results showed that the three strains of DYNDL21-2, DYNDL1-2 and FJNDD18M8 with sucrose metabolism capacity showed good growth and acid production ability. After fermentation, the abundance of hexanal and various furan heterocyclic compounds was significantly down-regulated, which could effectively reduce the intensity of beany odor. The significant up-regulation of acetaldehyde, acetoin and 2,3-butanedione in soymilk fermented by strain FJNDD18M8 improved the milky and creamy flavor of the product, thereby improving the flavor of soymilk.

Key words: Lactococcus lactis subsp. Lactis, soymilk, fermentation characteristics, metabolome