关键词: QuEChERS, 静电场轨道阱质谱, 抗虫药, 消炎药, 激素药物

Abstract: A method for the determination of 21 veterinary drugs in cow and goat milk and milk powder was established by QuEChERS combined with ultra performance liquid chromatography-quadrupole/orbitrap mass spectrometry (UPLC-Q/Orbitrap MS). The optimum test method was determined by optimizing chromatographic conditions, mass spectrometry parameters and pre-treatment conditions. The samples were extracted by acetonitrile salt-out under ultrasonication, and then purified by n-propyl ethylenediamine(PSA) and C18. The target analytes were separated by Accucore RP-MS column (100 mm×2.1 mm, 2.7 μm), and determined by UPLC-Q/Orbitrap MS. 21 kinds of veterinary drugs showed good linear relationship in their linear range, and the correlation coefficient (r2) was greater than 0.99. The Limits of quantification (LOQ) were 0.5 μg/kg. The average recoveries at 0.5, 2 and 10 μg/kg were 67.2%-110.2%, and the relative standard deviation (RSD) was 3.6%-17.4% (n=6). The method is simple and sensitive, and suitable for simultaneous detection of 21 kinds of veterinary drugs in cow and goat milk and milk powder.

Key words: QuEChERS, Orbitrap mass spectrometry, antiparasitics, antiphlogistics, glucocorticoids