关键词: β-乳球蛋白（β-LG）, 二十二碳六烯酸（DHA）, 互作效应, 致敏性

Abstract: β-lactoglobulin (β-LG) is widely recognized as a major allergen in cow's milk, and about 82.0% of cow's milk-allergic people have strong allergic reactions to β-LG. In this experiment, molecular simulation docking method, synchronous fluorescence spectroscopy, three-dimensional fluorescence spectroscopy, circular dichroism and Elisa method were used to investigate the interaction effect of Docosahexaenoic Acid (DHA) with β-LG and its influence on the allergenicity of β-LG. The results showed that DHA binds to β-LG mainly through hydrophobic forces and forms stable complexes. And the binding of DHA to β-LG changed the secondary structure of β-LG, in which the β-turn content of β-LG increased by about 30%. Meanwhile, the polarity of β-LG increased and hydrophobicity was weakened after binding, and the sensitizing property was reduced by about 30%. This study provides a theoretical basis for the development of hypoallergenic dairy products by analyzing the influence of the interaction effect between DHA and β-LG on their allergenicity.

Key words: β-lactoglobulin (β-LG), Docosahexaenoic Acid (DHA), interdependence effect, allergenicity