Abstract: An analytical method was established to determine fructooligosaccharides (FOS) in milk and milk products by high performance ion exchange chromatography method with pulsed amperometric detection. The column was a CarboPac PA1 (4 mm × 250 mm) anion exchange column. Fructooligosaccharides were separated under gradient elution mode. The mobile phase A was 150 mmol/L NaOH and the mobile phased B was composed of 150 mmol/L NaOH and 100 mmol/L sodium acetate. The relative standard derivations for 6 replicate determinations of four fructooligosaccharides in liquid milk were less than 9%. The average spike recovery rates for three fructooligosaccharides in liquid milk varied between 94.8% and 103.6%. The method was simple, fast and suitable for determining FOS in milk and milk products.

Key words: high performance ion exchange chromatography (HPIEC)；pulsed amperometric detection (PAD)； fructooligosaccharides (FOS)

摘要： 建立离子色谱法定量测定乳及乳制品中低聚果糖的方法,应用CarboPacTMPA1（4mm×250mm）阴离子交换柱、氢氧化钠和醋酸钠淋洗液梯度淋洗、脉冲安培检测器检测。6次测定液态奶中低聚果糖的含量,相对标准偏差小于9%,3种糖的加标回收率在94.8%～103.6%之间。本方法简单快捷,适用于乳及乳制品中低聚果糖含量的检测。

关键词: 高效离子色谱法;脉冲安培检测;低聚果糖