胆盐水解酶基因在干酪乳杆菌LC2W中的表达

doi:10.15922/j.cnki.jdst.2016.02.006

乳业科学与技术 ›› 2016, Vol. 39 ›› Issue (2): 21-24.DOI: 10.15922/j.cnki.jdst.2016.02.006

胆盐水解酶基因在干酪乳杆菌LC2W中的表达

黄艳娜, 刘振民, 游春苹

光明乳业股份有限公司,光明乳业研究院,乳业国家重点实验室,上海 200436

出版日期:2016-03-01 发布日期:2022-02-07
基金资助:
“十二五”国家科技支撑计划项目(2013BAD18B01,2013BAD18B02)

Overexpression of Bile Salt Hydrolase Genes in Lactobacillus casei LC2W

HUANG Yanna, LIU Zhenmin, YOU Chunping

(State Key Laboratory of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co. Ltd., Shanghai 200436, China)

Published:2016-03-01 Online:2022-02-07

摘要/Abstract

摘要： 从胆盐水解酶的特性出发,在干酪乳杆菌LC2W中过量表达Lactobacillus plantarum ST-Ⅲ 4种胆盐水解酶同工酶,通过酶活性的测定研究胆盐水解酶对干酪乳杆菌水解胆盐的作用.结果表明:实现了4种胆盐水解酶在干酪乳杆菌LC2W的过表达;利用茚三酮显色法测定的4种重组菌胆盐水解酶活性较宿主LC2W都不同程度地降低;在干酪乳杆菌LC2W中过量表达L.plantarum ST-Ⅲ的胆盐水解酶基因达不到提高该菌水解胆盐能力的目的.

关键词: 胆盐水解酶；干酪乳杆菌LC2W；克隆表达；酶活性

Abstract: In this study, the recombinant Lactobacillus casei LC2W were constructed for overexpressing four bsh genes from L. plantarum ST-III and the Bile salt hydrolase (BSH) enzyme activities were determined. It was shown that the four recombinant strains all showed a lower enzyme activity than the host, indicating that the bsh gene overexpression could not achieve the goal of enhancing the hydrolysis of the bile salts.

Key words: bile salt hydrolase; Lactobacillus casei LC2W; gene cloning and overexpression; enzyme activity

中图分类号:

Q556

黄艳娜, 刘振民, 游春苹. 胆盐水解酶基因在干酪乳杆菌LC2W中的表达[J]. 乳业科学与技术, 2016, 39(2): 21-24.

HUANG Yanna, LIU Zhenmin, YOU Chunping. Overexpression of Bile Salt Hydrolase Genes in Lactobacillus casei LC2W[J]. Journal of Dairy Science and Technology, 2016, 39(2): 21-24.

[1]	黄艳娜, 刘振民, 游春苹. 乳酸菌合成乳酸的研究现状[J]. 乳业科学与技术, 2016, 39(1): 19-23.
[2]	黄艳娜, 任婧. 胆盐水解酶基因结构与功能研究现状[J]. 乳业科学与技术, 2015, 38(2): 33-36.

胆盐水解酶基因在干酪乳杆菌LC2W中的表达

Overexpression of Bile Salt Hydrolase Genes in Lactobacillus casei LC2W

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