关键词: 肠道细菌基因间重复共有序列-聚合酶链式反应；指纹图谱技术；细菌分型；污染菌溯源

Abstract: Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) allows gene amplification using primers designed based on ERIC, which are widely present in living organisms, yielding products that are useful to characterize the genomic structure. This method has been widely used in bacterial classification and research on the genetic relationship of strains because of its merits such as simplicity, ease of implementation, good repeatability and time saving. This paper reviews the background to bacterial classification research and the principle and characteristics of ERIC-PCR technique, and summarizes the advantages and disadvantages of ERIC-PCR technique in bacterial genotyping and recent progress in its application to genotype common food-borne pathogenic bacteria. At the same time, future prospects for the application of ERIC-PCR in traceability analysis of microbial contaminants in dairy enterprises.

Key words: enterobacterial repetitive intergenic consensus-polymerase chain reaction; fingerprinting technology; bacterial typing; tracing of microbial contaminants