新疆双峰驼乳酪蛋白血管紧张素转化酶抑制肽的酶法制备和抑制特性

doi:10.15922/j.cnki.jdst.2021.03.005

乳业科学与技术 ›› 2021, Vol. 44 ›› Issue (3): 24-30.DOI: 10.15922/j.cnki.jdst.2021.03.005

新疆双峰驼乳酪蛋白血管紧张素转化酶抑制肽的酶法制备和抑制特性

刘宸, 王学清, 豆智华, 王俊, 李荣蓉, 徐赵玉, 杨洁

（新疆大学生命科学与技术学院，新疆乌鲁木齐 830046）

出版日期:2021-05-01 发布日期:2021-11-01
基金资助:
新疆维吾尔自治区重点研发计划项目（2018B01003）

Enzymatic Preparation and Activity Evaluation of Angiotensin Converting Enzyme Inhibitory Peptide from Xinjiang Bactrian Camel Milk Casein

LIU Chen, WANG Xueqing, DOU Zhihua, WANG Jun, LI Rongrong, XU Zhaoyu, YANG Jie

(College of Life Science and Technology, Xinjiang University, ürümqi 830046, China)

Published:2021-05-01 Online:2021-11-01

摘要/Abstract

摘要： 对新疆双峰驼乳酪蛋白分别进行胃蛋白酶和胰蛋白酶的单酶和双酶联合水解，用反相高效液相色谱外标法，对产生的马尿酸含量进行检测，测定水解产物血管紧张素转化酶（angiotensin converting enzyme，ACE）抑制活性。通过米式方程对比水解产物与卡托普利之间的竞争关系，并用50、10、3 kDa的超滤膜对水解液进行超滤，测定截留液ACE抑制活性。结果表明：驼乳酪蛋白单酶水解12 h过程中，胰蛋白酶水解4 h，水解度达到3.7%，胃蛋白酶水解4 h，水解度达到16.58%，胰蛋白酶水解2 h，水解

关键词: 血管紧张素转化酶, 抑制肽, 驼乳, 酪蛋白, 降血压

Abstract: The casein of the milk of bactrian camels in Xinjiang was hydrolyzed with pepsin and/or trypsin, and the angiotensin converting enzyme (ACE) inhibitory activity of the resulting hydrolysates was determined by measuring the production of hippuric acid (HA) by reversed-phase high performance liquid chromatography (RP-HPLC) using an external standard method. The competitive relationship between the casein hydrolysates and captopril was investigated by using the Michaelis-Menten equation. The ACE inhibitory activity of the retenates obtained after ultrafiltration of the hydrolysates through membranes with molecular mass cutoff of 50, 10 and 3 kDa was determined. The results showed that the degree of hydrolysis was 3.7% after 4 h hydrolysis with trypsin compared to 16.58% with pepsin. The percentage of ACE inhibition by the 2 h hydrolysate with trysin was 74.67% as opposed to 72.33% with pepsin. When the casein was hydrolyzed sequentially with pepsin for 2 h followed by trypsin for 4 h, the degree of hydrolysis increased in a linear manner with hydrolysis time. The percentage of ACE inhibition by the hydrolysate within 2 h reached 66.27%, which did not increase with hydrolysis time. Trypsin may act on the bioactive peptides, thus reducing the enzyme activity. At increasing substrate concentration, the hydrolysate could not counteract the ACE inhibitory effect of the polypeptides, which was characteristics of non-competitive inhibitors. The inhibitory constant Ki of the polypeptides was 0.25 mg/mL. The ACE inhibitory activity of the 3–10 kDa retenate was the highest, and the peptides LLVVYPWTR and VLPVPQQMVPYPQR were found to be structurally similar to the known ACE inhibitory peptides.

Key words: Keywords: angiotensin converting enzyme, inhibitory peptide, camel milk, casein, lowering blood pressure

中图分类号:

TS252.1

刘宸, 王学清, 豆智华, 王俊, 李荣蓉, 徐赵玉, 杨洁. 新疆双峰驼乳酪蛋白血管紧张素转化酶抑制肽的酶法制备和抑制特性[J]. 乳业科学与技术, 2021, 44(3): 24-30 https://doi.org/10.15922/j.cnki.jdst.2021.03.005

LIU Chen, WANG Xueqing, DOU Zhihua, WANG Jun, LI Rongrong, XU Zhaoyu, YANG Jie. Enzymatic Preparation and Activity Evaluation of Angiotensin Converting Enzyme Inhibitory Peptide from Xinjiang Bactrian Camel Milk Casein[J]. Journal of Dairy Science and Technology, 2021, 44(3): 24-30 https://doi.org/10.15922/j.cnki.jdst.2021.03.005

[1]	杨新月，曹双瑜，赵艳坤，姜娜，木尼拉·地里夏提，武亚婷，马宪兰，陈贺. 基于分散液-液微萃取技术的驼乳中外源牛胰岛素快速检测[J]. 乳业科学与技术, 2026, 49(2): 30-36.
[2]	周莹，孙万成，罗毅皓. 不同杀菌方式对驼乳稳定性及酪蛋白结构的影响[J]. 乳业科学与技术, 2026, 49(1): 23-32.
[3]	马希，刘清清，陈瑾，李斌，张颖，曹炜，赵东林，孙秋勃，彭德举. 富硒生物转化对羊乳酪蛋白组成及品质特性的影响[J]. 乳业科学与技术, 2025, 48(4): 1-9.
[4]	闫菩薇，张苗，米兰，王莹，宋雪梅，张炎. Mozzarella干酪拉伸特性的影响因素研究进展[J]. 乳业科学与技术, 2025, 48(4): 54-61.
[5]	和金泽，崔浩然，李钰芳，郑文涛，彭清雅，李丽，黄艾祥. 中甸牦牛乳酪蛋白源生物活性肽的鉴定及抗氧化活性[J]. 乳业科学与技术, 2024, 47(6): 1-7.
[6]	乔巍，许倩，王宇，韩雪，吕晓红，侯爱菊. 酪蛋白磷酸肽提高矿物质元素生物利用度的研究进展[J]. 乳业科学与技术, 2024, 47(5): 32-38.
[7]	梅芷晴，马浩睿，刘永峰，胡坚，舒琴. 羊乳母乳化及主要活性成分研究进展[J]. 乳业科学与技术, 2024, 47(4): 38-46.
[8]	徐杏敏，郑远荣，刘振民，苏米亚. 红曲霉代谢产物的抗感染与代谢调节功能研究进展[J]. 乳业科学与技术, 2024, 47(4): 53-58.
[9]	陈琪，张秀荣，何静，吉日木图. 热处理条件对驼乳乳清蛋白理化性质和功能性的影响[J]. 乳业科学与技术, 2023, 46(4): 1-9.
[10]	郭红蕾, 周胜云, 丛艳君, 闫文杰. 茶多酚对牛乳过敏原αs1-酪蛋白构象和抗原性的影响[J]. 乳业科学与技术, 2022, 45(4): 7-13.
[11]	谭宏凯, 程剑锋, 熊子奕, 胡巍, 李欣, 陈红兵. 消减牛乳酪蛋白致敏性蛋白酶的筛选[J]. 乳业科学与技术, 2022, 45(4): 22-28.
[12]	魏家祺, 周胜云, 丛艳君, 闫文杰. 热加工方式对牛乳过敏原αs1-酪蛋白二级结构和抗原性的影响[J]. 乳业科学与技术, 2022, 45(4): 29-35.
[13]	宋卓岩, 乔春艳, 张雪茹, 郝果, 刘永峰. 基于反相高效液相色谱技术测定市售羊乳粉中蛋白[J]. 乳业科学与技术, 2022, 45(4): 52-56.
[14]	钟丽雯, 鲁迎瑞, 张卫兵, 郭兆斌, 张金亮, 文鹏程. 补铁剂及多肽铁复合物的研究进展[J]. 乳业科学与技术, 2022, 45(4): 63-69.
[15]	兰丽, 张彩霞, 高万东, 余志宝, 徐红, 唐振鑫, 范敏, 杨明阳, 田加美, 张雪娇, 张晶, 贺晓鸣, 郭素芳. 液相色谱法测定超高温灭菌乳中蛋白含量[J]. 乳业科学与技术, 2022, 45(2): 18-23.

新疆双峰驼乳酪蛋白血管紧张素转化酶抑制肽的酶法制备和抑制特性

Enzymatic Preparation and Activity Evaluation of Angiotensin Converting Enzyme Inhibitory Peptide from Xinjiang Bactrian Camel Milk Casein

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics