关键词: 高效液相色谱法；牛乳；灭蝇胺；代谢产物；三聚氰胺

Abstract: A high performance liquid chromatography method was established to determine the content of cyromazine and its metabolite melamine in milk. The external standard method was used to quantify figures of merit such as linear relationship, precision, recovery, detection limit, and quantification limit. The results showed that the method had good linearity in the concentration range of 0.0–1.0 mg/L (R2 > 0.999), with detection limits of 0.003 and 0.004 mg/kg and quantification limits of 0.008 and 0.010 mg/kg for cyromazine and melamine, respectively. The average spiked recoveries were in the range of 84.4% to 89.5% and 83.9% to 91.2% for cyromazine and melamine, respectively, with relative standard deviations (RSDs) less than 10%. This method is characterized by high sensitivity, high accuracy, and good reproducibility, and can meet the requirement of the determination of cyromazine and its metabolite melamine in milk.

Key words: high performance liquid chromatography; milk; cyromazine; metabolites; melamine