超高效液相色谱法同时测定配方乳粉中的烟酸、烟酰胺和VB6

doi:10.7506/rykxyjs1671-5187-20240116-003

摘要/Abstract

摘要： 建立一种能够同时测定配方乳粉中烟酸、烟酰胺和VB6的超高效液相色谱（ultra performance liquid chromatography，UPLC）分析方法。以10 mmol/L乙酸铵溶液（含0.1%甲酸）为流动相A，甲醇为流动相B进行梯度洗脱，将Poroshell 120 EC-C18与ACQUITY UPLC? HSS T3色谱柱串联分离目标物，经紫外检测器和荧光检测器检测，用外标法定量。结果表明：烟酸质量浓度为0.0～0.8 μg/mL、烟酰胺质量浓度为0.0～20.0 μg/mL、吡哆胺质量浓度为0.0～0.2 μg/mL、吡哆醛质量浓度为0.0～0.6 μg/mL，以及吡哆醇质量浓度为0.0～4.0 μg/mL，5 种化合物标准曲线线性良好，相关系数均大于0.999，检出限和定量限均符合要求；该方法测定的平均回收率为80.5%～104.1%，相对标准偏差为0.37%～2.99%。该分析方法简便、准确，可进行配方乳粉中烟酸、烟酰胺和VB6的批量测定。

关键词: 配方乳粉；烟酸；烟酰胺；VB6；超高效液相色谱法；检测

Abstract: To establish an ultra performance liquid chromatography (UPLC) analysis method that can simultaneously determine niacin, niacinamide, and vitamin B6 in formula milk powder. Gradient elution was performed with 10 mmol/L ammonium acetate solution (containing 0.1% formic acid) as mobile phase A and methanol as mobile phase B. The analytes were separated by Poroshell 120 EC-C18 in tandem with ACQUITY? UPLC HSS T3 column, detected with the UV detector and fluorescence detector, and quantified by an external standard method. Good linearity was observed for niacin concentrations in the range of 0.0-0.8 μg/mL，nicotinamide concentrations in the range of 0.0-20.0 μg/mL, pyridoxamine concentrations in the range of 0.0-0.2 μg/mL, pyridoxal concentrations in the range of 0.0-0.6 μg/mL and pyridoxine concentrations in the range of 0.0-4.0 μg/mL, and the correlation coefficients of standard curves were all greater than 0.999. The limits of detection and quantification met the requirements. The average recoveries of the method were all in the range of 80.5%-104.1% with relative standard deviation (RSD) were 0.37%-2.99%. This method is simple, accurate, can be used for batch detection of niacin, niacinamide, and vitamin B6 in formula milk powder.

Key words: formula milk powder; niacin; niacinamide; vitamin B6; ultra performance liquid chromatography; determination

中图分类号:

TS252.7

崔苏霞，孟根花，蔺婷娟，李晓燕. 超高效液相色谱法同时测定配方乳粉中的烟酸、烟酰胺和VB6[J]. 乳业科学与技术, 2023, 46(6): 29-33.

CUI Suxia, MENG Genhua, LIN Tingjuan, LI Xiaoyan. Simultaneous Determination of Niacin, Nicotinamide, and Vitamin B6 in Formula Milk Powder by Ultra Performance Liquid Chromatography[J]. Journal of Dairy Science and Technology, 2023, 46(6): 29-33.

[1]	黄建辉，张彦辉，张耀广，赵翠琴. 牛乳中β-乳球蛋白的中红外快速检测方法建立[J]. 乳业科学与技术, 2025, 48(1): 14-19.
[2]	李娇，李巧莲，周长民，袁帅，罗景阳，陈娇，丁婷. 超高效液相色谱-串联质谱法同时测定婴幼儿配方乳粉中5 种脂溶性维生素[J]. 乳业科学与技术, 2025, 48(1): 20-23.
[3]	王胜杰，梁琳. 气相色谱-质谱法测定婴幼儿配方乳粉中3-氯-1,2-丙二醇酯和缩水甘油酯[J]. 乳业科学与技术, 2024, 47(6): 31-36.
[4]	徐志鹏，泮秋立，肖晶，张爽，宋家升，王骏. 胶体金免疫层析试纸条鉴别乳品中牛、羊源性成分的性能评价[J]. 乳业科学与技术, 2024, 47(6): 37-42.
[5]	杨琦，焦昕哲，孟虎，房忠芳，张耀武，赵丽莉，张倩婷. QuEChERS处理-超高效液相色谱-串联质谱法快速检测鲜乳中54 种农药残留[J]. 乳业科学与技术, 2024, 47(6): 43-50.
[6]	白晓云，马晓冲，齐晓茹，苏运聪，张耀广，李飞. 固相萃取-气相色谱-质谱法测定牛乳中54 种农药残留[J]. 乳业科学与技术, 2024, 47(5): 12-19.
[7]	周帅康，胡连霞，王孟阳，李苗，卢涵，王世杰. 基于实时荧光定量环介导等温扩增技术快速检测发酵乳中嗜热链球菌和保加利亚乳杆菌[J]. 乳业科学与技术, 2024, 47(3): 9-15.
[8]	梁建英，唐烁，刘丽君，黄晓燕，岳虹，胡文慧，李翠枝. 乳及乳制品中蛋白酶检测技术研究进展[J]. 乳业科学与技术, 2024, 47(3): 80-85.
[9]	祁兵，陈柔含，杨晓君，江春铃，马颖清，邓波. 基于生物芯片技术的生乳中兽药检测方法验证及其应用[J]. 乳业科学与技术, 2024, 47(2): 42-48.
[10]	刘予煊，孟根花，蔺婷娟，郭瑞芳. 婴幼儿配方乳粉中1,2-丙二醇检测方法的优化[J]. 乳业科学与技术, 2024, 47(2): 49-55.
[11]	王海涛, 沈潇, 姚凌云, 孙敏, 王化田, 冯涛. 采用GC×GC-TOFMS分析3 种脂肪含量牛乳中挥发性化合物[J]. 乳业科学与技术, 2024, 47(1): 26-32.
[12]	崔苏霞. 超高效液相色谱法测定乳粉中烟酰胺含量的不确定度评定[J]. 乳业科学与技术, 2024, 47(1): 33-37.
[13]	刘玲，叶沛，张巧艳，申颖，董玉英，蒋孟圆，李文廷. 高效液相色谱-串联质谱法测定乳及乳制品中17种真菌毒素[J]. 乳业科学与技术, 2023, 46(5): 22-27.
[14]	岳虹，唐烁，宁月莲，黄晓燕，胡文慧，梁建英，刘丽君，李翠枝. 高效液相色谱法测定发酵乳中甜菊糖苷[J]. 乳业科学与技术, 2023, 46(5): 28-34.
[15]	黄晓燕，唐烁，刘丽君，李翠枝，宁月莲，岳虹. 乳及乳制品中元素检测技术研究进展[J]. 乳业科学与技术, 2023, 46(5): 61-67.

超高效液相色谱法同时测定配方乳粉中的烟酸、烟酰胺和VB6

Simultaneous Determination of Niacin, Nicotinamide, and Vitamin B6 in Formula Milk Powder by Ultra Performance Liquid Chromatography

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics