An HPLC method to determine dicyandiamide in milk and dairy products was proposed and validated in the present study. A comparative investigation of gradient and isocratic elution was carried out for the chromatographic separation of dicyandiamide. The results of this study indicated a good linear relationship for dicyandiamide over the concentration range of 0.1002 to 10.0200 μg/mL under gradient elution conditions (r = 0.9996). The average recovery of dicyandiamide was 92.8%, and the limit of detection was 0.01 μg/mL. This method proved to be applicable for the determination of dicyandiamide in milk and dairy products.