Abstract: An HPLC method to determine dicyandiamide in milk and dairy products was proposed and validated in the present study. A comparative investigation of gradient and isocratic elution was carried out for the chromatographic separation of dicyandiamide. The results of this study indicated a good linear relationship for dicyandiamide over the concentration range of 0.1002 to 10.0200 μg/mL under gradient elution conditions (r = 0.9996). The average recovery of dicyandiamide was 92.8%, and the limit of detection was 0.01 μg/mL. This method proved to be applicable for the determination of dicyandiamide in milk and dairy products.

Key words: dicyandiamide；milk powder；dairy products；high performance liquid chromatography (HPLC)； determination

摘要： 用高效液相色谱(HPLC)梯度法(不含盐流动相)和等度法(含盐流动相)测定奶粉及乳制品中双氰胺的含量.通过实验对2种方法进行比较,确立适合奶粉及乳制品中双氰胺的检测方法,并进行方法学验证.结果表明:梯度法测定双氰胺在0.1002～10.0200μg/mL范围内线性良好(r=0.9996);平均回收率92.8％,最低检测限为0.01μg/mL,可用于奶粉及乳制品中双氰胺含量的测定.

关键词: 双氰胺；奶粉；乳制品；高效液相色谱法；含量测定