Abstract: Create a method for simultaneously measuring the levels of vitamins A, D3, K2, E, and β Carotene in infant formula milk powder using ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The five soluble in fat The five fat soluble vitamins have a good linear relationship within the concentration range of 1-800 ng/mL. The relative standard deviation ranges from 0.4% to 2.6%, while the recovery rates for high, medium, and low concentrations are between 92% and 108%. The limit of quantification of this method is12.5~125μg/100g, the sample pre-treatment steps are simple, the sensitivity of quantitative analysis is high, and the accuracy and precision of the detection results are good.

Key words: ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), Infant Formula Milk Powder, Fat-soluble Vitamin

摘要： 建立超高效液相色谱-串联质谱法（high performance liquid chromatography-tandem mass spectrometry，UPLC-MS/MS）同时测定婴幼儿配方乳粉中维生素A、D3、K2、E、β胡萝卜素。5种脂溶性维生素在1~800ng/mL浓度范围内的线性关系良好。高、中、低三水平浓度的加标回收率在92%~108%，相对标准偏差在0.4%~2.6%。该方法的定量限为12.5~125μg/100g，样品前处理的步骤简单，定量分析的灵敏度高，检测结果的准确性和精密度良好。

关键词: 超高效液相色谱-串联质谱法, 婴幼儿配方乳粉, 脂溶性维生素