Abstract: Lactobacillus paracasei subsp. paracasei LC-01 is a probiotic strain which has strong antioxidant and immunoregulatory activities. This study was designed to evaluate the laxative effect of strain LC-01 in constipated mice. Male Balb/c mice were randomly divided into blank group, model control group, positive control group and three dose groups. Both the blank and model control groups were administered orally with 10% skim milk at a dose of 0.1 mL/(kg·d) once a day. Lactobacillus casei subsp. casei Shirota (1 × 108 CFU/mL) and Lactobacillus casei subsp. casei LC-14 (1 × 106, 1 × 108 CFU/mL and 1 × 1010 CFU/mL) were used as control strains (also at 0.1 mL/(kg·d)). Strain LC-01 suspensions at concentrations of 1 × 106, 1 × 108 CFU/mL and 1 × 1010 CFU/mL were given to mice at the same dose as 10% skim milk. Constipation was induced in mice by compound diphenoxylate. After 7 days of administration of strain LC-01, the first defecation time of mice was shortened, and the number, weight and water content of stools were increased markedly. After another 7 days, the ink propelling rate became much higher. These results indicate that strain LC-01 has an outstanding laxative effect in constipated mice.

Key words: Lactobacillus paracasei；defecation；mice；constipation

摘要： 副干酪乳杆菌LC-01是一株具有较强抗氧化活性和免疫活性的益生菌菌株,为评价该菌株的润肠通便作用,实验通过将Balb/c雄性小鼠随机分为空白组、模型组、LCS对照组和3个副干酪乳杆菌LC-01给药组.空白组和模型组经口灌胃0.1mL/(kg·d)(以体质量计)质量分数10％的脱脂乳.选取代田乳酸菌LCS及干酪乳杆菌菌株LC-14作为对照菌株.代田乳酸菌LCS对照组灌胃1×108CFU/mL的菌悬液0.1mL/(kg·d),LC-14对照组和LC-01给药组分别相应给予低、中、高剂量(1×106、1×108、1×1010CFU/mL)的活菌液0.1mL/(kg·d).使用复方地芬诺酯建立小鼠便秘模型,副干酪乳杆菌LC-01给药7d后,便秘小鼠首次排便时间缩短,排便粒数和质量明显增加,粪便水分含量提高:连续15d灌胃副干酪乳杆菌LC-01,便秘小鼠小肠墨汁推进率也得到显著增加.结果表明,副干酪乳杆菌LC-01具有良好的润肠通便功能.

关键词: 副干酪乳杆菌; 通便; 小鼠; 便秘