Abstract: Probiotic Lactobacillus strains were isolated from yogurt, cultured on de Man, Rogosa and Sharpe (MRS) agar and identified according to their 16S rRNA sequences. The Lactobacilli strains were assessed for hydrogen peroxide (H2O2) production and adhesion to HeLa cells to determine their potential for preventing infections of the female reproductive tract. A total of 10 isolates were identified including 5 L. acidophilus strains, 1 L. paracasei strain, 1 L. helveticus strain, 1 L. rhamnosus strain, 1 L. delbrueckii strain and 1 L. plantarum strain. The L. helveticus strain was strongly adherent to HeLa cells and demonstrated high H2O2 production, whereas other 9 strains had a relatively low ability to produce H2O2. Although most of the probiotic Lactobacillus strains isolated from yogurt did not have biochemical characteristics in relation to preventing infections of the female reproductive tract, the L. helveticus strain might have a protective effect on female urogenital health.

Key words: Lactobacillus；yogurt；adhesion；HeLa cell；H2O2

摘要： 对国内外市售酸奶中添加的乳酸杆菌益生菌进行分离鉴定,对其产H2O2量及与宫颈癌HeLa细胞黏附能力进行分析,并初步判定酸奶中的乳酸杆菌是否对女性生殖道健康具有潜在的保健作用.实验共分离鉴定出10株乳酸杆菌,其中,嗜酸性乳杆菌5株、干酪乳杆菌1株、瑞士乳杆菌1株、鼠李糖乳杆菌1株、植物乳杆菌1株和德式乳杆菌1株.只有瑞士乳杆菌产H2O2量较高且与HeLa细胞的黏附作用较强,其余乳酸杆菌产H2O2的能力偏低.大部分国内外市售酸奶产品中添加的益生菌不具备抑制女性生殖道感染有关的生理生化特性,只有瑞士乳杆菌可能对女性生殖系统的健康具有保护作用.

关键词: 乳杆菌; 酸奶; 黏附; HeLa细胞; 过氧化氢