Abstract: Sixty milk samples were collected from dairy cows with mastitis in Shanghai to study the antimicrobial resistance of Staphylococcus aureus. Staphylococcus aureus isolation, identification and antimicrobial resistance were investigated. Eight Staphylococcus aureus isolates (8/60, 13.33%) were obtained from the collected samples. The antimicrobial resistance test revealed that 100.00% of the isolates were resistant to penicillin G and ampicillin as well as cefalotin, cefmetazole and cefuroxime sodium, 50.00% were resistant to sulfamethoxazole, 37.50% were resistant to cefaclor, ceftizoxime, lomefloxacin, norfloxacin, tetracycline, gentamicin, erythromycin, clindamycin and chloramphenicol, 25.00% were resistant to ciprofloxacin and ofloxacin, and 12.50% were resistant to cefotaxime, ceftazidime and enoxacin. Four Staphylococcus aureus isolates had multi-resistance abilities.

Key words: Staphylococcus aureus; mastitis; antimicrobial resistance

摘要： 为调查上海地区奶牛乳房炎金黄色葡萄球菌的耐药性，采集上海地区乳房炎患病乳样60 份，进行金黄色葡萄球菌的分离鉴定及药敏实验。结果表明：经分离鉴定，共检出8 株金黄色葡萄球菌，分离率为13.33%；药敏实验结果表明，分离得到的金黄色葡萄球菌对青霉素G和氨苄西林的耐药率最高，均为100.00%，对磺胺甲恶唑的耐药率次之，为50.00%，对头孢克洛、头孢唑肟、洛美沙星、诺氟沙星、四环素、庆大霉素、红霉素、克林霉素和氯霉素的耐药率均为37.50%，对环丙沙星和氧氟沙星的耐药率均为25.00%，对头孢噻肟、头孢他啶

关键词: 金黄色葡萄球菌；乳房炎；耐药性