关键词: 超高效液相色谱-串联质谱法, 牛奶, 奶制品, β-受体激动剂

Abstract: A method for the determination of 25 β-receptor agonists in milk and dairy products was established based on ultra-high performance liquid chromatography-tandem mass spectrometry.The samples were enzymolized in ammonium acetate solution for 16 h, fat and protein were removed by glass fiber filter paper,andpurified by PRIME MCX solid phase extraction column.The separation was performed using Waters BEH C18 column (100 mm ×2.1 mm, 1.7 μm). Acetonitrile and 0.1% formic acid water were used as mobile phase for gradient elution. Electrospray ion source was used, positive ion multiple reaction monitoring was performed, and quantitative determination was performed by internal standard method. The results showed that the linearity of the three vanillin analogues was good in the concentration range of 0.2~50ng/mL with the correlation coefficients (R2) greater than 0.996; the recoveries ranged from 80.3% to 106.2% with the relative standard deviations (RSDs) of 0.7%~8.5% (n=6). The method is simple, efficient and suitable for the determination of 25 β-receptor agonists in milk and dairy products.

Key words: ultra high performance liquid chromatography, milk, Dairy Product, β-receptor agonists