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乳业科学与技术 ›› 2019, Vol. 42 ›› Issue (6): 1-5.DOI: 10.15922/j.cnki.jdst.2019.06.001

• 基础研究 •    下一篇

绵羊乳与其他乳种营养成分及乳清蛋白组分差异性分析

吴仪凡, 葛武鹏, 刘凯茹, 宋宇轩, 赵丽丽, 张文博, 王雨馨, 梁秀珍   

  1. 西北农林科技大学食品科学与工程学院, 陕西杨凌, 712100, 陕西省羊乳产品质量监督检验中心, 陕西富平, 711700, 陕西中检检测技术有限公司, 陕西西安, 710068, 陕西飞天乳业有限公司, 陕西宝鸡, 721100
  • 出版日期:2019-11-01 发布日期:2021-11-09
  • 基金资助:
    西北农林科技大学金昌奶绵羊试验示范基地建设项目（A289021806）；陕西省创新能力支撑计划项目（2017XY-02）

Analysis of Nutritional Composition and Whey Protein Composition of Sheep Milk: A Comparison withMilk from Humans and Other Mammals

WU Yifan, GE Wupeng, LIU Kairu, SONG Yuxuan, ZHAO Lili, ZHANG Wenbo, WANG Yuxin, LIANG Xiuzhen   

  1. (1.College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China; 2.Shaanxi Goat Milk Product Quality Supervision and Inspection Center, Fuping 711700, China; 3.Shaanxi Zhongjian Test Technology Co.Ltd., Xi’an 710068, China; 4.Shaanxi Feitian Dairy Co. Ltd., Baoji 721100, China)
  • Online:2019-11-01 Published:2021-11-09

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摘要： 为研究绵羊乳与其他乳种之间营养成分及乳清蛋白组分的差异性，以山羊乳、牛乳、人乳为对照，分析不 同泌乳阶段的湖羊乳、东佛里生羊乳及其杂交一代羊乳的乳清蛋白组成差异，利用十二烷基硫酸钠-聚丙烯酰胺凝 胶电泳（sodium dodecyl sulfate-polyacrylamide gel electropheresis，SDS-PAGE）和非变性-PAGE（Native-PAGE）分 析标志性差异蛋白。结果表明：不同品种、不同泌乳阶段绵羊乳之间以及不同乳种之间的蛋白质、脂肪、乳糖等宏 量营养素含量存在显著差异（P＜0.05），含量变化呈现趋势性；SDS-PAGE分析表明，绵羊乳和人乳的标志性差 异蛋白为β-乳球蛋白和乳铁蛋白，绵羊乳和山羊乳的标志性差异蛋白为α-乳白蛋白，绵羊乳和牛乳的标志性差异蛋 白为免疫球蛋白G重链；Native-PAGE分析表明，绵羊乳与山羊乳乳清蛋白之间存在1 条差异性条带，绵羊乳与人 乳、牛乳乳清蛋白之间存在3 条差异性条带。

关键词: 绵羊乳；营养成分；乳清蛋白；十二烷基硫酸钠-聚丙烯酰胺凝胶电泳；非变性聚丙烯酰胺凝胶电泳

Abstract: The purpose of this study was to explore the differences in nutritional components and whey protein components between sheep milk and milk from humans and other animal species used for dairy production. The whey protein components of milk from Hu sheep, East Friensian Milk sheep and their F1 hybrid at different stages of lactation were analyzed and compared with those of goat, cow and human milk. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Native-PAGE were used to identify landmark proteins that differed between milk samples. The contents of milk macronutrients like protein, fat and lactose significantly varied between sheep breeds, between lactation stages, and between sheep and other animal species (P < 0.05) and they showed a certain trend. SDS-PAGE showed that β-lactoglobulin and lactoferrin differed between sheep and human milk, α-lactalbumin differed between sheep and goat milk, and immunoglobulin G (IgG) heavy chain differed between sheep and cow milk. Native-PAGE indicated that there was one protein band that differed between sheep and goat whey as well as three ones that differed between sheep milk and human and cow whey.

Key words: sheep milk; nutritional composition; whey proteins; sodium dodecyl sulfate-polyacrylamide gel electropheresis; native-polyacrylamide gel electropheresis

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