发酵乳后熟期间嗜热链球菌和保加利亚乳杆菌产乙醛特性

doi:10.15922/j.cnki.jdst.2014.01.001

乳业科学与技术 ›› 2014, Vol. 37 ›› Issue (1): 1-5.DOI: 10.15922/j.cnki.jdst.2014.01.001

• 生物技术 •

发酵乳后熟期间嗜热链球菌和保加利亚乳杆菌产乙醛特性

白娜, 于洁, 王宏梅, 乌仁图雅, 秦艳婷, 扎木苏, 刘文俊, 孟和毕力格, 张和平

内蒙古农业大学乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特,010018

发布日期:2022-02-11
基金资助:
国家高技术研究发展计划(863计划)项目(2011AA100902)

Acetaldehyde-Producing Properties of Streptococcus thermophilus and Lactobacillus bulgaricus in Fermented Milk during Post-Ripening

<a href="http://www.dairyst.net.cn/EN/article/advancedSearchResult.do?searchSQL=(((ZHA Mu-su[Author]) AND 1[Journal]) AND year[Order])" target="_blank">ZHA Mu-su</a>,<a href="http://www.dairyst.net.cn/EN/article/advancedSearchResult.do?searchSQL=((( QIN Yan-ting[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> QIN Yan-ting</a>,<a href="http://www.dairyst.net.cn/EN/article/advancedSearchResult.do?searchSQL=((( WU REN Tu-ya[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> WU REN Tu-ya</a>,<a href="http://www.dairyst.net.cn/EN/article/advancedSearchResult.do?searchSQL=((( REN Yan[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> REN Yan</a>,<a href="http://www.dairyst.net.cn/EN/article/advancedSearchResult.do?searchSQL=((( WANG Xue-ni[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> WANG Xue-ni</a>,<a href="http://www.dairyst.net.cn/EN/article/advancedSearchResult.do?searchSQL=((( LIU Wen-jun[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> LIU Wen-jun</a>,<a href="http://www.dairyst.net.cn/EN/article/advancedSearchResult.do?searchSQL=((( MENG HE Bilige[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> MENG HE Bilige</a>,<a href="http://www.dairyst.net.cn/EN/article/advancedSearchResult.do?searchSQL=((( ZHANG He-ping[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> ZHANG He-ping</a>

(Key Laboratory of Dairy Biotechnology and Bioengineer, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China)

Published:2022-02-11

摘要/Abstract

摘要： 目的:分析嗜热链球菌和保加利亚乳杆菌在发酵乳后熟期间产乙醛特性,并研究乙醛合成与其关键调控基因表达量之间的关系.方法:以传统发酵乳制品中筛选出的具有优良发酵特性的嗜热链球菌和保加利亚乳杆菌为研究对象,将各试验菌株在复原全脂乳中进行单菌发酵,发酵结束后(pH4.5～4.6)于4℃冷藏后熟,测定48 h内发酵乳中的乙醛含量;采用反转录定量PCR技术检测乙醛合成关键调控基因pdc、pdh、ald、ldh的表达特征.结果:6株嗜热链球菌产乙醛量介于2.59～14.53 μg/g之间,6株保加利亚乳杆菌产乙醛量介于9.17～39.45 μg/g之间;乙醛合成量随着基因pdc、ald及pdh表达量的升高而增加,而与基因ldh的表达量呈负相关.结论:发酵乳后熟期间嗜热链球菌和保加利亚乳杆菌不同菌种、不同菌株乙醛产量差异显著,相同菌株在不同后熟时间产乙醛量差异明显,存在菌株特异性和时序变异性;调控基因pdc、pdh及ald具有促进乙醛合成的作用,基因ldh的表达不利于乙醛含量的积累.

关键词: 发酵乳；嗜热链球菌；保加利亚乳杆菌；乙醛；调控基因

Abstract: The aim of this study was to analyze the acetaldehyde-producing characteristics of Streptococcus thermophilus and Lactobacillus bulgaricus in fermented milk during post-ripening and to investigate the relationship between the expression of key regulatory genes and acetaldehyde synthesis. Methods: Fermented milk was obtained from single strain fermentation of reconstituted whole milk by potent strains of S. thermophilus and L. bulgaricus isolated from traditional fermented milk products. After the end of fermentation (pH 4.5-4.6), the fermented milk was ripened by storage at 4 ℃ for 48 h. During the storage period, the change in acetaldehyde content was determined, and the expression characteristics of the key regulatory genes pdc, pdh, ald, and ldh involved in acetaldehyde synthesis were examined by reverse transcriptionqPCR. Results: The amounts of acetaldehyde produced by 6 S. thermophilus strains were 2.59–14.53 μg/g and 9.17–39.45 μg/g by 6 Lactobacillus bulgaricus strains. The synthesis of acetaldehyde was positively correlated with the expression levels of pdc, ald and pdh but negatively correlated with the expression level of ldh. Conclusion: Different bacterial species and different strains have significantly different capacities to produce acetaldehyde during post-ripening, and one strain produces significantly different amounts of acetaldehyde at different post-ripening times, suggesting strain and temporal specificity of acetaldehyde production. The regulatory genes pdc, ald, and pdh promote the synthesis of acetaldehyde, while the gene ldh has a negative effect on acetaldehyde production.

Key words: fermented milk; Streptococcus thermophilus; Lactobacillus bulgaricus; acetaldehyde; regulatory gene

中图分类号:

TS252.54

白娜, 于洁, 王宏梅, 乌仁图雅, 秦艳婷, 扎木苏, 刘文俊, 孟和毕力格, 张和平. 发酵乳后熟期间嗜热链球菌和保加利亚乳杆菌产乙醛特性[J]. 乳业科学与技术, 2014, 37(1): 1-5.

ZHA Mu-su, QIN Yan-ting, WU REN Tu-ya, REN Yan, WANG Xue-ni, LIU Wen-jun, MENG HE Bilige, ZHANG He-ping. Acetaldehyde-Producing Properties of Streptococcus thermophilus and Lactobacillus bulgaricus in Fermented Milk during Post-Ripening[J]. Journal of Dairy Science and Technology, 2014, 37(1): 1-5.

[1]	许英瑞, 吴世芳, 杨晓丽, 薛元泰, 朱妍丽, 张卫兵, 赵军, 文鹏程. 桦褐孔菌多糖对牦牛酸乳品质的影响[J]. 乳业科学与技术, 2021, 44(3): 12-18.
[2]	许英瑞, 朱妍丽, 马瑞娟, 赵军, 薛元泰, 张卫兵, 张炎, 文鹏程. 活性多糖在发酵乳中的应用研究进展[J]. 乳业科学与技术, 2021, 44(2): 26-30.
[3]	洪青, 刘振民. 发酵乳中生物活性肽的研究进展[J]. 乳业科学与技术, 2021, 44(2): 31-36.
[4]	徐显睿, 李翠凤, 张宗博, 夏文洋, 隋勇军, 李道河, 陈政言, 张兰威. 乳酸杆菌抑制致病菌及生物保鲜发酵剂特性评价[J]. 乳业科学与技术, 2021, 44(1): 18-22.
[5]	徐显睿, 李翠凤, 隋勇军, 张宗博, 杨洪来, 李道河, 陈政言, 张兰威. 响应面法优化乳双歧杆菌Z-1冷冻干燥保护剂配方[J]. 乳业科学与技术, 2020, 43(3): 6-11.
[6]	徐显睿, 李翠凤, 杨洪来, 隋勇军, 李道河, 张宗博, 陈政言, 张兰威. 具有益生潜力的乳酸杆菌筛选[J]. 乳业科学与技术, 2020, 43(2): 8-12.
[7]	谈文诗, 欧凯, 刘冠辰, 王健, 冯玉红, 吴伟都. 高蛋白饮用型酸乳体系中微粒化乳清蛋白和物理改性淀粉对稳定性的影响[J]. 乳业科学与技术, 2020, 43(1): 1-4.
[8]	齐世华, 张栋, 赵林森, 艾连中, 王世杰. 西藏灵菇中Lactobacillus casei LC577的分离鉴定及特性[J]. 乳业科学与技术, 2019, 42(5): 16-20.
[9]	王健, 欧凯, 常云鹤, 李罗飞, 王银娟, 高兴华. 长保质期酸乳关键加工过程对变性淀粉的影响[J]. 乳业科学与技术, 2016, 39(4): 18-21.
[10]	张冬蕾, 陈红霞, 德亮亮, 刘文俊, 孙天松. 内蒙古鄂尔多斯地区传统发酵乳制品中乳酸菌优势菌群q-PCR定量分析[J]. 乳业科学与技术, 2015, 38(6): 1-5.
[11]	王健, 张佳佳, 王银娟, 李罗飞, 常云鹤, 欧凯, 高兴华. 3种变性淀粉的糊化特性及其在长保质期酸乳中的应用[J]. 乳业科学与技术, 2015, 38(5): 1-5.
[12]	任江红, 李龙柱, 秦立虎, 王攀. 乳成分对凝固型酸乳贮藏品质的影响[J]. 乳业科学与技术, 2015, 38(4): 14-17.
[13]	陈红霞, 德亮亮, 任艳, 张冬蕾, 杨彦荣, 刘文俊, 张和平. 嗜热链球菌产酸特性及其发酵乳中有机酸组分分析[J]. 乳业科学与技术, 2015, 38(2): 1-5.
[14]	娄新曼, 王存芳. 红枣羊乳啤的制备及其稳定性[J]. 乳业科学与技术, 2015, 38(2): 15-19.
[15]	王银娟, 王晓君, 田芬, 孙勇, 欧凯, 吴伟都, 高兴华. 乳清浓缩蛋白改善脱脂发酵乳饮料的稳定性[J]. 乳业科学与技术, 2014, 37(6): 1-5.

发酵乳后熟期间嗜热链球菌和保加利亚乳杆菌产乙醛特性

Acetaldehyde-Producing Properties of Streptococcus thermophilus and Lactobacillus bulgaricus in Fermented Milk during Post-Ripening

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics