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乳业科学与技术 ›› 2020, Vol. 43 ›› Issue (2): 8-12.DOI: 10.15922/j.cnki.jdst.2020.02.002

• 加工工艺 • 上一篇    下一篇

具有益生潜力的乳酸杆菌筛选

徐显睿, 李翠凤, 杨洪来, 隋勇军, 李道河, 张宗博, 陈政言, 张兰威   

  1. 青岛根源生物技术集团有限公司食品实验室, 山东 青岛 266000;青岛诺和诺康实业有限公司, 山东 青岛 266000, 青岛诺和诺康实业有限公司, 山东 青岛 266000, 中国海洋大学食品科学与工程学院, 山东 青岛 266000
  • 出版日期:2020-03-01 发布日期:2021-11-02

Screening of Lactobacillus for Probiotic Potential

XU Xianrui, LI Cuifeng, YANG Honglai, SUI Yongjun, LI Daohe, ZHANG Zongbo, CHEN Zhengyan, ZHANG Lanwei   

  1. (1.Food Laboratory of Qingdao Genyuan Biotechnology Group Co. Ltd., Qingdao 266000, China; 2. Qingdao GBW Industrial Co. Ltd., Qingdao 266000, China; 3.College of Food Science and Engineering, Ocean University of China, Qingdao 266000, China)
  • Online:2020-03-01 Published:2021-11-02

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摘要： 以分离自西藏、新疆、青海地区传统自然发酵样品中的30 株可用于食品中的乳酸杆菌为研究对象，以鼠李糖乳杆菌LGG为对照，经耐酸、耐胆盐、疏水作用、静电作用和自聚能力评价，初步筛选获得16 株乳酸菌，对初筛优良菌株进行模拟胃液和模拟肠液耐受性实验。结果表明：4 株菌在模拟胃液中孵育60 min存活率大于14.44%，4 株菌在模拟胃液中孵育30 min存活率为3.91%～100.00%；16 株菌在模拟肠液中孵育5 h存活率为31.73%～112.83%；菌株Z-1为最佳抗性菌株，在模拟胃液中孵育60 min存活率为93.07%，在模拟肠液中孵育5 h存活率为106.5%，疏水率为97.52%，对氯仿和乙酸乙酯的静电作用率为97.35%和24.67%，自凝聚率为11.26%。

关键词: 乳酸杆菌, 益生潜力, 疏水作用, 静电作用, 自聚能力, 模拟胃液, 模拟肠液

Abstract: A total of 30 food-grade Lactobacillus strains isolated from naturally fermented products from Tibetan, Xinjiang and Qinghai regions including Lactobacillus rhamnosus LGG (as a control) were evaluated for acid and bile salt tolerance, hydrophobicity, surface charge and auto-aggregation ability. Out of these, 16 strains were selected and further evaluated for tolerance to artificial gastric and intestinal fluids. The results showed that the survival rates of 4 of the 16 strains were greater than 14.44% after 60 min of incubation in artificial gastric fluid and were 3.91%–100.00% after 30 min. For all 16 selected strains, the survival rate was 31.73%–112.83% after 5 h, and Z-1 had the strongest tolerance to artificial gastric fluid of these strains with a 93.07% survival rate after 60 min. The survival rate of Z-1 was 106.5% after 5 h of incubation in artificial intestinal fluid, hydrophobicity 97.52%, surface charge 97.35% and 24.67% in the presence of chloroform and ethyl acetate, respectively, and auto-aggregation ability 11.26%.

Key words: Lactobacillus, probiotic potential, hydrophobicity, surface charge ability, auto-aggregation ability, artificial gastric juice, artificial intestinal juice

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