关键词: 保加利亚乳杆菌;嗜热链球菌;碳源;氮源;生长因子

Abstract: ：An enrichment culture medium has been developed for the industrial mass production of lactic acid bacteria. Onefactor- at-a-time experiments were conducted to choose optimal carbon and nitrogen sources and growth factor, and their levels were optimized using an orthogonal array design. Our results demonstrated that the optimal medium composition consisted of 10% pineapple peel juice, 25% Pleurotus ostreatus soup and 20% potato juice. The enrichment culture medium was not only significantly more effective in enriching lactic acid bacteria than MRS medium but also inexpensive, thus favoring the industrial mass production of lactic acid bacteria.

Key words: Lactobacillus bulgaricus；Streptococcus thermophilus；carbon source；nitrogen source；growth factors