Abstract: A high performance liquid chromatography method was established to determine the content of cyromazine and its metabolite melamine in milk. The external standard method was used to quantify figures of merit such as linear relationship, precision, recovery, detection limit, and quantification limit. The results showed that the method had good linearity in the concentration range of 0.0–1.0 mg/L (R2 > 0.999), with detection limits of 0.003 and 0.004 mg/kg and quantification limits of 0.008 and 0.010 mg/kg for cyromazine and melamine, respectively. The average spiked recoveries were in the range of 84.4% to 89.5% and 83.9% to 91.2% for cyromazine and melamine, respectively, with relative standard deviations (RSDs) less than 10%. This method is characterized by high sensitivity, high accuracy, and good reproducibility, and can meet the requirement of the determination of cyromazine and its metabolite melamine in milk.

Key words: high performance liquid chromatography; milk; cyromazine; metabolites; melamine

摘要： 建立高效液相色谱法测定牛乳中灭蝇胺及其代谢产物三聚氰胺含量的分析方法。采用外标法对线性关系、精密度、回收率、检出限以及定量限等指标定量。结果表明，该方法在0.0～1.0 mg/L线性良好（R2＞0.999），灭蝇胺、三聚氰胺检出限分别为0.003、0.004 mg/kg，定量限分别为0.008、0.010 mg/kg，平均加标回收率分别为84.4%～89.5%、83.9%～91.2%，相对标准偏差均小于10%。该研究方法具有灵敏、准确、重现性好等特点，能满足牛乳中灭蝇胺及其代谢产物三聚氰胺的测定需求。

关键词: 高效液相色谱法；牛乳；灭蝇胺；代谢产物；三聚氰胺