A method was developed for the simultaneous determination of 2 ultraviolet absorbers UV-326, UV-1577 and 2 antioxidants AO-1010 and AO-2246 in milk by high performance liquid chromatography. Samples were extracted with acetonitrile. The effective separation of the target analytes was accomplished on Agilent HC-C8 (150 mm × 4.6 mm, 5.0 μm) column with gradient elution procedure using a mixture of water and acetonitrile as the mobile phase. External calibration was used for quantitative determination at detection wavelength of 280 nm. The linearity of 4 analytes were satisfactory with a correlation coefficient of > 0.9995 at concentrations ranging from 0.05 to 50 μg/mL. The limits of detection of the method were 0.02?0.5 mg/kg, and the recoveries at different spiked levels and precision (RSD) were 69.08%?115.87% and 0.43%?9.94%, respectively. The method is simple, sensitive, reproducible and suitable for the simultaneous determination of 2 ultraviolet absorbers and 2 antioxidants in milk.