Lactulose as a prebiotic is widely used in milk-based products, especially in infant formula as an important component. For regulation, the Chinese national standard Food Additive-Lactulose Liquid GB8816—1988 has been relatively backward. And there is no regulation regarding the analysis of lactulose in foods. How to discriminate lactulose from other carbohydrates, especially from lactose, is a problem we are faced with and an obstacle for application. The chromatographic techniques for the determination of lactulose in milk-based products, including thin-layer chromatography, gas chromatography and liquid chromatography, are summarized. This work could provide a reference for lactulose detection in milk-based product by researchers.