Kombucha, also known as Haibao, is a traditional acidic tea beverage fermented by a symbiotic culture of lactic acid bacteria, acetic acid bacteria and yeast. Lactic acid bacteria (LAB) were isolated and purified from kombucha and the purified strains were identified. The results showed that a total of 4 LAB strains (XC, XD, XL and XQ) were isolated and they were all identified as Lactobacillus plantarum. These strains entered the stationary growth phase after 14 h. For all four strains, both the pH value and titratable acidity declined during their growth. The optimal inoculum size for XC, XD and XQ was 4%, while that for XL was 6%. The optimal growth temperature for these strains was 30 ℃ and none of them was resistant to salts. All these strains had different tolerance to bile salts and acidity and XQ was the most tolerant among them.