An efficient method was developed for the simultaneous analysis of six estrogens including diethylstilbestrol, α-estradiol, β-estradiol, estriol, estrone and bisphenol A in milk powder using gas chromatography-tandem mass spectrometry (GC-MS-MS). Samples were enzymatically hydrolyzed and extracted with acetonitrile. The extract was then derivatized with pentafluoropropionic anhydride before being injected into the GC-MS-MS system. Qualitative and Quantitative analysis was carried out under the multiple reaction monitoring (MRM) mode. The results indicated that all six estrogens displayed a good linear relationship with R2 ＞ 0.999. The limits of quantification for bisphenol A and the other five estrogens were 0.03 and 0.3 μg/kg, respectively. The average recovery of six estrogens was 93.93%, with RSD of 6.05%, meeting the methodological requirements. The method developed in this study can be successfully applied in the determination of six estrogens in milk powder.