Donkey milk is considered as an ideal substitute for human milk because of its high contents of whey protein, lactose, lysozyme, unsaturated fatty acids and vitamin C, and low contents of casein and fat. Additionally, donkey milk has various physiological functions, including hypoallergenic, bacteriostatic and anticancer activity. Thus, this review introduces readers to the nutritional composition of donkey milk, including proteins, amino acids, fats, minerals, vitamins., and compares it with that of human milk, bovine milk, buffalo milk, goat milk, and camel milk, in order to provide useful information for the comprehensive processing and utilization of donkey milk.