The applicability of enzyme colorimetry as specified in the National Food Safety Standard: Determination of Choline in Infant Food and Milk Products (GB 5413.20－2013) for the determination of choline in infant formula for special medical purposes was evaluated using quality control and actual samples as well as spiked recovery and precision tests. The theoretical value of choline content in quality control samples was 171.5 mg/100 g, and the measured value was 168.4 mg/100 g. The measured result was within the allowable error range indicating its reliability. Spiked recoveries for infant formula and quality control samples were 96.1%–98.4% with relative standard deviation (RSD) of 1.43%, indicating high accuracy and precision of the method. When the method was applied to 24 batches of commercial samples, the measured values of choline content met the requirements of the national standard. Accordingly, enzyme colorimetry can be used to detect choline i n infant formula for special medical purposes.