This research evaluated the stability of vitamins and active components under ultra-low temperature conditions in order to provide a basis to formulate the quality standard for breast milk bank to ensure minimal nutrient loss during storage at ?80 ℃. The results showed that vitamin contents significantly reduced with storage time up to 12 months, yet no significant change was observed in nucleotide contents. After 3 months of storage, the contents of VB2 and VA decreased to 35% and 29% of the original level, respectively, while VC was at undetectable level. Moreover, nicotinic acid at the end of storage dropped to 69% of the fresh breast milk. Considering the existing quality indicators, the quality standard for breast milk bank should be based on breast milk components to be analyzed and fresh breast milk should be used as far as possible to analyze ingredients with poor stability such as vitamins.