According to the opinions of the state administration for market supervision on standardizing the use of rapid food detection, the performance of commercial colloidal gold immunochromatographic test strips for rapid in situ identification of bovine-, ovine- and caprine-derived ingredients in dairy products were evaluated in terms of application scope, detection limit, cross-reaction rate, sensitivity, false negative rate, false positive rate and coincidence rate with the results of the reference method. The results showed that the application scope of the test strip for bovine-derived ingredients was raw milk, and the detection limit was 5% (V/V); the application scope of the test strip for ovine- and caprine-derived ingredients was raw ovine and caprine milk, and the detection limit was 0.3% (V/V). Both test strips had no cross-reaction with enrofloxacin, sulfamethazine, dexamethasone, tetracycline or aflatoxin M1. The sensitivity, false positive rate and false negative rate of the test strips were 100%, 0% and 0%, respectively. The coincidence rate with the results of the reference method was high. In conclusion, the colloidal gold immunochromatographic test strips have high accuracy, and can be used for rapid in situ screening of adulterated milk.

Given the increasing focus on the safety of milk and milk products, this study set up a database application program using the target analytes included in the Codex standards for milk and milk products as search path, which can provide technical support to achieve network informatization of work on food safety and a technical platform to achieve resource sharing, thereby ensuring people’s health and safety. In addition, the present study can also facilitate further research on food safety standards for both domestic monitoring and international trade.