Objective: To investigate the effects of two lactic acid bacteria (LAB) preparations (pure cultures of Bifidobacterium animalis No.6 and Bifidobacterium animalis.subsp.lactis Bl Qq08) on plasma lipid levels of hyperlipidemic rats. Methods: SD rats were fed high fat diet to establish preventive hyperlipidemia models. SD rats were divided into the following groups: normal control, model control, low, middel and high dose Bifidobacterium animalis No.6, and low, middel and high dose Bl Qq08. After oral administration for 45 consecutive days, the effects of both LAB preparations on serum triglyceride (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-c) in hyperlipidemic rat models were observed. Results: Compared with normal control group, serum TG and TC in model control group increased significantly (P＜0.01) after 45-days high-fat feeding. Compared with model control group, serum TG and TC in low dose group of Bifidobacterium animalis No.6 fell significantly (P＜0.05), and the two indicators exhibited an extremely significant decrease in middle dose and high dose group of Bifidobacterium animalis No.6. Consistent results were found when comparing different dose groups of Bl Qq08 and model control group. Howeve, no significant difference in serum HDL-c existed among three dose groups for each LAB prepration. Conclusion: Both LAB preprations help reduce blood lipids.