Abstract: Probio+,, malt extract, which is extracted from barely, is rich in natural nutrients. An HPLC method was used to determine oligosaccharides in Probio+,, malt extract in this study. Meanwhile, the effect of different amounts of Probio+,, malt extract addition on probiotic growth in yoghurt during storage was tested by spread plate method. The results showed that the contents of cellotriose and cellotetraose in Probio+,, malt extract were 10.45μg/mg and 2.79μg/mg, respectively. The addition of Probio+,, malt extract at 3% and sucrose at 4% indicated the best growth promoting effect on probiotics (Bulgaria lactobacillus, Streptococcus thermophilus and Bifidobacterium) in yoghurt, which was better than fructo-oligosaccharide at the same levels.

Key words: Probio+,, malt extract；cellotriose；cellotetrose；yogurt

摘要： 采用高效液相色谱法检测保益加麦精中纤维低聚糖含量,并在酸奶制作过程中添加不同比例的保益加麦精,采用平板涂布的方法检测不同储存时间下,检测保益加麦精对酸奶益生菌的生长及储存过程中的影响。结果测得保益加麦精中纤维三糖、四糖含量分别为10.45、2.79μg/mg,当保益加麦精添加量3%、蔗糖添加量4%时,对酸奶中益生菌（保加利亚乳杆菌、嗜热链球菌及双歧杆菌）的促生长作用最好,优于同等添加量的低聚果糖。

关键词: 保益加麦精; 纤维糖含量; 酸奶