Abstract: Whey proteins, as high-quality proteins in milk, have rich in nutritional value and functional properties. It can vary in expression and structure due to genetic variation and post-translational modification of proteins in species under different environments, making them diverse and complex, which makes in-depth understanding of whey proteins more difficult. With the continuous development of omics techniques, a deeper understanding of the composition and functional activities of whey proteins has been achieved. This paper reviewed the application of proteomics in the study of bovine whey proteins, and analyzed the similarities and differences between the types, molecular structures, metabolic pathways, and biological functions of whey proteins in different breeds of cows, lactation periods, feeding conditions,and processing methods with a view to providing scientific theoretical basis for the in-depth study of bovine whey proteins and the development of personalized dairy.

Key words: Key words: Proteomics, Bovine whey protein, Molecular structure, Metabolic pathway, Biological function

摘要： 乳清蛋白作为乳中的优质蛋白，具有丰富的营养价值和功能特性。乳清蛋白会因不同环境下物种的遗传变异和蛋白翻译后修饰导致表达量和结构的差异，使得其种类繁多而又复杂，给深入了解乳清蛋白增加了难度。随着组学技术的不断发展，人们对乳清蛋白的组成和功能活性有了更深入了解。本文综述蛋白质组学技术在牛乳清蛋白研究中的应用，解析不同牛种、泌乳期、饲养条件和处理方式下乳清蛋白的种类、分子结构、代谢途径及生物功能之间的异同，以期为牛乳清蛋白的深入研究及个性化乳制品的研制提供科学的理论依据。

关键词: 关键词：蛋白质组学, 牛乳清蛋白, 分子结构, 代谢途径, 生物功能