Abstract: To identify and test the quality of commercial goat milk powder sold in different areas, reversed-phase high performance liquid chromatography (RP-HPLC) was used to determine the protein composition of 35 samples of pure and formulated goat milk powder purchased in different regions (Shaanxi and non-Shaanxi) of China and in other countries, and the difference in protein composition among goat milk powder from different production areas and that between pure and formulated goat milk powder were analyzed. The results showed that the contents of αs2-casein and β-casein in formulated milk powder were 20% and 24% lower than those in pure milk powder, respectively, and the contents of κ-casein and whey proteins were 30% and 50% higher than those in pure milk powder, respectively. However, the protein composition and content of pure goat milk powder from different regions were similar. Therefore, RP-HPLC is useful for protein quality evaluation of commercial goat milk powder.

Key words: reversed-phase high performance liquid chromatography; whey protein; casein; goat milk powder; identification

摘要： 为鉴别及检测不同地区市售羊乳粉的品质，基于反相高效液相色谱（reversed-phase high performance liquid chromatography，RP-HPLC）技术，将35 种市售羊乳粉根据陕西地区和非陕西地区、纯羊乳粉和配方羊乳粉进行分 类与检测，研究不同产地、不同种类羊乳粉的差异，对羊乳粉品质进行分析判别。结果表明：对市售羊乳粉乳蛋 白进行RP-HPLC测定发现，配方羊乳粉αs2-酪蛋白（αs2-casein，αs2-CN）和β-CN相对含量分别低于纯羊乳粉20%和 24%，κ-CN和乳清蛋白相对含量分别高于纯羊乳粉30%和50%；不同地区纯羊乳粉乳蛋白组成及含量相似。因此， 通过RP-HPLC可以实现对市售羊乳粉中乳蛋白的测定和质量评价。

关键词: 反向高效液相色谱；乳清蛋白；酪蛋白；羊乳粉；鉴定