Abstract: In order to obtain more data on proteins in goat milk, cow milk and human milk, their basic components were determined, and bioinformatic analysis of their proteins was performed by proteomics. The results showed that the protein contents of goat milk and cow milk were higher than that of human milk, while the contents of fat and lactose were higher in human milk. A total of 3 370 proteins were identified in the three kinds of milk, and gene ontology (GO) analysis of these proteins indicated that they were mainly involved in biological processes such as cell process and metabolism process, and distributed in the cell, organelles and extracellular region. Their molecular functions included binding and catalytic activity. The KEGG pathway varied in the three kinds of milk and was enriched in protein processing in the endoplasmic reticulum. In the protein-protein interactions network, the 307 shared proteins participated in several pathways such as carbon metabolism, glycolysis/gluconeogenesis, and amino acid biosynthesis.

Key words: goat milk, cow milk, human milk, ultra performance liquid chromatography tandem mass spectrometry, proteomics

摘要： 为进一步了解羊乳、牛乳和人乳3 种乳样蛋白质相关信息，对其基本成分进行测定，并通过蛋白质组学技术对所含蛋白质进行生物信息学分析。结果表明：羊乳和牛乳的蛋白质含量高于人乳，人乳的脂肪和乳糖含量较高；3 种乳中共鉴定出3 370 种蛋白质，乳中蛋白质的生物过程主要集中在细胞和代谢过程，细胞定位主要分布在腔体和细胞器上，分子功能表现为结合功能和催化活性；KEGG通路在3 种乳中富集程度和种类有明显差异，共有的高富集通路为蛋白质在内质网的加工过程；蛋白质相互作用网络中的共有蛋白质（307 种）主要集中在碳代谢、糖

关键词: 羊乳, 牛乳, 人乳, 超高效液相色谱-串联质谱, 蛋白质组学