Abstract: The composition and contents of oligosaccharides in two mixed samples of human breast milk were determined and the effect of the human milk oligosaccharides (HMOs) 2’-fucosyllactose (2’-FL) on the proliferation of two probiotic strains, Bifidobacterium Bb-12 and Lactobacillus fermentum CECT5716 was evaluated in this study. The results showed that the HMOs contents of the two mixed samples were 10.613 and 5.093 g/L respectively. This significant difference indicated that HMOs contents in breast milk from different mothers are affected by many factors, generally ranging between 5 and 15 g/L. 2’-FL promoted the growth of both priobiotics, indicating that the probiotics could utilize HMOs for growth and proliferation.

Key words: human milk, human milk oligosaccharides, proliferation of probiotics, Bifidobacterium, Lactobacillus fermentum

摘要： 研究混合母乳中复杂的母乳低聚糖（human milk oligosaccharides，HMOs）组成及含量，选择双歧杆菌Bb-12、发酵乳杆菌CECT5716 2 种益生菌，研究HMOs 2’-岩藻糖（2’-fucosyllactose，2’-FL）对2 种益生菌的增殖作用。结果表明：2 个混合样本的HMOs含量分别为10.613、5.093 g/L，差异很大，说明不同母乳来源的HMOs受诸多因素影响，但含量范围一般为5～15 g/L；2’-FL对发酵乳杆菌CECT5716和双歧杆菌Bb-12均有

关键词: 母乳, 母乳低聚糖, 益生菌增殖, 双歧杆菌, 发酵乳杆菌