Abstract: Human milk oligosaccharides (HMO) are important bioactive ingredients of breast milk, the most abundant being 2’-fucosyllactose (2’-FL), which is significantly beneficial to the growth and development of infants, and is considered as a novel nutrient supplement with great potential. Recently, great progress has been made in the bio-production of 2’-FL, whose output has reached industrial levels of production. Many multi-national companies have investigated 2’-FL bio-production techniques for a long time. However, universities and companies in China have just begun research in this field, highlighting its great significance. This paper reviews the 2’-FL bio-production pathways and summarizes several recent novel 2’-FL biosynthesis methods.

Key words: human milk oligosaccharide, breast milk, 2’-fucosyllactose, cell factory, bioengineering

摘要： 人乳寡糖是母乳的重要活性成分，其中2’-岩藻糖基乳糖（2’-fucosyllactose，2’-FL）占比最高，对婴幼儿生长发育效果明显，是极具潜力的新型营养强化剂。近年来，2’-FL的生物合成方法取得长足进步，产量已经达到产业化水平，国外很多跨国公司均开始布局2’-FL的制备技术，国内高校和企业尚处于起步阶段，因此针对该项技术的探讨与研究具有重要意义。本文对2’-FL的生物制造途径进行综述，总结近年来报道的几种2’-FL合成新方法。

关键词: 人乳寡糖, 母乳, 2’-岩藻糖基乳糖, 细胞工厂, 生物工程