Abstract: Objective: To assess the safety of 3 enterococci strains (LQ-R-16-1, FC-3-7 and FC-3-2) originating from milk in mice through 7-day acute oral toxicity test and 30-day feeding test. Methods: Kunming mice of clean grade were subjected to intragastric administration of different doses of enterococci and during the test, the mice were observed for changes in physical signs and daily weight gain; afterwards, they were anatomized to observe organ changes and bacterial translocation and calculate organ indices. Results: Acute toxicity and feeding tests exhibited no significant difference in daily weight gain or organ indices compared with the control group (P > 0.05), and after the 30-day feeding test bacterial translocation was found not to occur. Conclusion: Enterococci LQ-R-16-1, FC-3-7, FC-3-2 are safe to consume, having no toxic side effects.

Key words: lactic acid bacteria; acute toxicity; 30-day feeding

摘要： 通过小鼠的7d急性毒性实验及30 d喂养实验,对源自牛乳中的3株肠球菌LQ-R-16-1、FC-3-7、FC-3-2进行安全性初步评价.分别对清洁级昆明小白鼠进行7d及30d连续灌胃不同剂量的肠球菌LQ-R-16-1、FC-3-7、FC-3-2,实验期间观察小鼠体征变化、计算日增体质量,最后通过大体解剖,观察脏器变化、脏器细菌易位情况并称质量计算脏器指数,脏器细菌易位情况.结果表明:7d急性毒性实验及30d喂养实验中各实验组小鼠日增体质量、脏器指数与对照组之间无显著性差异(P＞0.05),30 d喂养实验结束后未发现脏器之间有细菌易位现象.初步表明,肠球菌LQ-R-16-1、FC-3-7、FC-3-2安全,无毒副作用.

关键词: 乳酸菌；急性毒性；30d喂养