Abstract: high performance liquid chromatographic method was established to determine native and denatured α-lactalbumin in infant formula milk powder. Guanidine hydrochloride buffer was used as both sample extraction solution and HPLC mobile phase. Beta-mercaptoethanol was used as reductant for sample pretreatment. The chromatographic separation was performed on a TSK-GEL G3000SWXL column and the pooled eluate was detected at 280 nm. The quantification was carried out using breast milk as internal standard. Good separation and accurate determination of α-lactalbumin in infant formula milk powder was completed using the method in around 20 min. The method was fast, accurate, and suitable for the determination of α-lactalbumin in infant formula milk powder.

Key words: α-lactalbumin；high performance liquid chromatography；infant formula milk powder

摘要： 目的:建立高效液相色谱(HPLC)方法检测婴幼儿配方乳粉中变性和非变性的α-乳白蛋白.方法:使用盐酸胍缓冲液作为样品溶解液和HPLC流动相,用巯基乙醇作为还原试剂对样品进行前处理,以母乳样品为内标,使用TSK-GEL G3000SWXL柱,用紫外检测法在280nm波长处对洗脱物进行分析.结果:此方法可以准确测定喷雾干燥工艺加工后婴幼儿配方乳粉中的α-乳白蛋白含量,且分离效果好,分析所用的时间约为20min.结论:该方法快速、准确,适用于婴幼儿配方乳粉中α-乳白蛋白的检测.

关键词: α-乳白蛋白; 高效液相色谱(HPLC); 婴幼儿配方乳粉