Abstract: The objective of this study was to determine the growth curves and antibacterial activity of two different strains of Lactobacillus salivarius. Streptococcus mutans, Porphyromonas gingivalis and Staphylococcus aureus were selected to perform antibacterial tests in vitro by the Oxford cup method. The results showed that the logarithmic growth phase of the two strains of Lactobacillus salivarius began respectively at 2 and 4 hours after inoculation and ended both at 8 hours, and the stationary phase began at 14 and 16 hours, respectively. It was proved that the bacteria cultures of both strains of Lactobacillus salivarius and their metabolites had antibacterial activity against Streptococcus mutans, Porphyromonas gingivalis and Staphylococcus aureus, and the antibacterial activity against Streptococcus mutans and Porphyromonas gingivalis was stronger than that against Staphylococcus aureus. Also, for both strains, neither bacterial sludge nor metabolite with an adjusted pH value (7.0) had antibacterial activity. Antibacterial activity of both strains of Lactobacillus salivarius came mainly from their metabolites, and it were acid or acid-dependent materials that play a key role in the antibacterial activity of metabolite.

Key words: Lactobacillus salivarius; growth curve; Oxford cup method; antibacterial activity

摘要： 为了探讨唾液乳杆菌的生长特性及抑菌作用,测定2株唾液乳杆菌的生长曲线,并选用口腔变形链球菌、牙龈卟啉单胞菌及金黄色葡萄球菌为指示菌,采用牛津杯法进行体外抑菌实验.结果表明:2株唾液乳杆菌分别在接种2h和4h进入对数生长期,8h进入对数生长末期,14h和16h进入稳定期;2株唾液乳杆菌菌液及代谢产物对口腔变形链球菌、牙龈卟啉单胞菌及金黄色葡萄球菌均有抑菌作用,对口腔变形链球菌及牙龈卟啉单胞菌的抑菌作用强于金黄色葡萄球菌;2株唾液乳杆菌菌体及调整pH值为7.0的代谢产物没有抑菌作用;2株唾液乳杆菌的抑菌能力主要来自菌体的代谢产物,代谢产物中起抑菌作用的成分为酸或酸依赖性物质.

关键词: 唾液乳杆菌；生长曲线；牛津杯法；抑菌作用